Anti-NANOG Rabbit Polyclonal Antibody for WB, ELISA - Q80Z64

Core Product Specifications and Parameters

Biological Background: NANOG Function and Localization

• Homeobox protein NANOG, also known as ES cell-associated protein 4, is a transcription regulator crucial for inner cell mass and embryonic stem (ES) cell proliferation and self-renewal. Related references: PMID:25825768
• Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages.
• Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with coactivator recruitment.
• Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' and can act as either a transcriptional activator or repressor.
• Able to autorepress its expression in differentiating ES cells by binding to its own promoter following interaction with ZNF281/ZFP281, leading to NuRD complex recruitment.
• Localizes to the nucleus and is not expressed in many somatic organs, oocytes, spermatogonia, or hematopoietic cell lines at the protein level.
• Alternative splicing produces multiple isoforms, and the protein features a homeobox DNA-binding domain; associated keywords include developmental protein, activator, repressor, and DNA-binding.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 160-305 of mouse NANOG, a region that may contribute to the observed human cross-reactivity; consider sequence alignment between mouse and human NANOG to assess epitope conservation.
• For Western blot (WB), the predicted molecular weight is approximately 34 kDa; include appropriate positive controls such as mouse embryonic stem cell lysates and validate detection specificity using knockdown or knockout samples if available.
• In ELISA applications, use recombinant NANOG protein or cell extracts as coating antigens and empirically determine optimal antibody dilutions; cross-reactivity with human NANOG allows potential use in human sample testing with appropriate validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutions a streamlined, one-stop channel for sourcing high-quality IVD raw materials like this NANOG polyclonal antibody, suited for stem cell and developmental biology applications. Our extensive portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, complemented by rare target sourcing capabilities. Whether you need single components or integrated supply solutions, we bridge the gap from concept to clinic with technical expertise and reliable logistics.