Anti-Nanog Monoclonal Antibody for WB, IF-P, ELISA - Q9H9S0

Core Product Specifications and Parameters

Biological Background: Homeobox protein NANOG Function and Localization

• NANOG (Homeobox protein NANOG) is a transcription regulator essential for inner cell mass and embryonic stem cell proliferation and self-renewal. It imposes pluripotency on ES cells and prevents differentiation towards extraembryonic endoderm and trophectoderm lineages.
• Acts as a transcriptional activator or repressor, binding optimally to DNA consensus sequences 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed:25825768). Related references: PMID:25825768
• Blocks BMP-induced mesoderm differentiation by physically interacting with SMAD1, interfering with coactivator recruitment to SMAD transcriptional complexes.
• When overexpressed, promotes cells to enter S phase and proliferation.
• Contains a homeobox DNA-binding domain and a C-terminal transactivation domain; the protein has a molecular weight of ~34.6 kDa (canonical sequence).
• Localizes predominantly to the nucleus (SUBCELLULAR LOCATION: Nucleus).
• Tissue expression is restricted to testicular carcinoma, germ cell tumors, fetal gonads, ovary, and testis; not detected in many somatic organs and oocytes (TISSUE SPECIFICITY).
• Keywords: Alternative splicing, developmental protein, DNA-binding, homeobox, nucleus, reference proteome, repeat, repressor, transcription, transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 153-305) spans part of the transactivation domain and may recognize both full-length and alternatively spliced isoforms. Validate isoform detection in your system.
• Expected band size in Western blotting is ~35 kDa. Use appropriate lysis buffers for nuclear extracts to ensure efficient detection.
• For immunofluorescence/immunocytochemistry (IF), expect nuclear staining pattern; counterstain with DAPI to confirm localization.
• Antibody is validated for ELISA; consider direct or sandwich ELISA formats for quantitative detection of Nanog in stem cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio provides validated antibody pairs, optimized monoclonal antibodies (such as this anti-Nanog clone), and bulk ancillary reagents to streamline your diagnostic and research workflows. With expertise in stem cell targets, we support procurement of rare specificities and can assist in sourcing custom raw materials for your unique assay development needs. Whether you require off-the-shelf or customized solutions, CamelBio bridges the gap from concept to clinic.