Anti-Na+/K+-ATPase Rabbit Polyclonal Antibody for WB and ELISA - P05023 / P50993 / P13637 / Q13733

Core Product Specifications and Parameters

Biological Background: Na+/K+-ATPase Subunit Alpha-1 Function and Localization

• The ATP1A1 gene encodes the alpha-1 subunit of the sodium/potassium-transporting ATPase (Na+/K+-ATPase), a P-type ATPase that serves as the catalytic core of the sodium pump.
• It catalyzes ATP hydrolysis to actively transport 3 Na+ out of the cell and 2 K+ into the cell per cycle, establishing and maintaining electrochemical gradients across the plasma membrane (PubMed:29499166, PubMed:30388404, PubMed:35803952). Related references: PMID:29499166, PMID:30388404, PMID:35803952
• The enzyme undergoes ATP-driven conformational changes that mediate alternating access for ion binding and release, enabling vectorial ion translocation (PubMed:35803952). Related references: PMID:35803952
• Located predominantly at the basolateral cell membrane, sarcolemma, and axon cell projections, as well as in melanosomes, reflecting specialized roles in polarized cells and neural function.
• May also participate in an osmosensory signaling pathway that senses body-fluid sodium levels and regulates salt intake and voluntary water intake to maintain sodium homeostasis (By similarity).
• Post-translational modifications include acetylation and phosphorylation; disease associations include Charcot-Marie-Tooth disease, epilepsy, and intellectual disability, underscoring its critical physiological roles.
• The protein has a molecular mass of approximately 112.9 kDa (P05023), with multiple isoforms generated by alternative splicing; metal ion binding (Mg2+, K+) is essential for its catalytic activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 386–595 of human Na+/K+-ATPase alpha-1 (NP_000692.2), a region that may be conserved in rat (cross-reactivity observed); validation in rodent models is recommended.
• For Western blotting, expect bands around 113 kDa for the primary human isoform (P05023), with additional bands possible due to other isoforms (see Protein Weight); optimize loading and transfer conditions accordingly.
• ELISA protocols should be titrated with the immunogen peptide or recombinant protein to determine sensitivity and specificity; consider paired antibody formats for sandwich assays.
• Storage and handling: product shipped on ice packs; aliquot and store at recommended temperatures to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including this Na+/K+-ATPase rabbit polyclonal antibody, suitable for diagnostic research focused on ion transport and membrane protein targets. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your assay development from concept to clinic. For rare or custom targets, our sourcing team can leverage global networks to deliver the specific raw materials you need.