Anti-Na+/K+-ATPase Rabbit Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P05023

Core Product Specifications and Parameters

Biological Background: Na+/K+-ATPase Function and Localization

• Encoding and protein identity: The alpha-1 subunit is encoded by the ATP1A1 gene in humans and serves as the catalytic core of the Na+/K+-ATPase pump.
• Core ion transport activity: It hydrolyzes ATP to drive the export of three Na+ ions and import of two K+ ions against their electrochemical gradients (PubMed:29499166, PubMed:30388404, PubMed:35803952). This process relies on ATP-driven conformational changes (PubMed:35803952). Related references: PMID:29499166, PMID:30388404, PMID:35803952
• Osmoregulatory role: Could participate in an osmosensory pathway that monitors sodium levels and regulates salt and water intake (By similarity).
• Subcellular distribution: Found in the basolateral cell membrane, sarcolemma, axon projections, and melanosomes, reflecting its role in polarized cells.
• Disease relevance: Mutations in ATP1A1 are associated with Charcot-Marie-Tooth disease type 2DD, primary hypomagnesemia, epilepsy, and intellectual disability.
• Post-translational modifications: Subject to acetylation and phosphorylation, which may modulate pump activity and trafficking.
• Structural insights: The protein features multiple transmembrane helices and nucleotide-binding domains; its 3D structure has been elucidated.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100); the antibody is expected to recognize the full-length alpha-1 subunit, but may not distinguish between functionally similar isoforms.
• For Western blotting, a predominant band near 113 kDa is anticipated; verify specificity using appropriate controls (e.g., knockout lysates or siRNA depletion).
• In IHC-P and IF applications, optimize antigen retrieval and permeabilization protocols; co-staining with basolateral or sarcolemmal markers can aid localization studies.
• The antibody’s cross-reactivity with mouse and rat enables use in common rodent models, but validate tissue-specific expression and band size predictions beforehand.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing comprehensive IVD raw-material solutions, connecting diagnostic manufacturers and laboratories with high-quality antibodies like this anti-Na+/K+-ATPase clone. Whether you need validated antibody pairs, bulk monoclonal reagents, or related ancillary products for ion transport and membrane biology research, CamelBio streamlines procurement from concept to clinic. Reach out today to discuss custom sourcing, technical support, or volume orders tailored to your development pipeline.