Anti-NAIP Rabbit Polyclonal Antibody for WB, ELISA - Q13075

Core Product Specifications and Parameters

Biological Background: NAIP Function and Localization

• NAIP (BIRC1/NLRB1) is a 160 kDa baculoviral IAP repeat-containing protein and member of the NLR family. It functions as an anti-apoptotic factor and a sensor component of the NLRC4 inflammasome.
• It inhibits apoptosis by suppressing the activities of CASP3, CASP7, and CASP9, preventing the autocleavage of pro-CASP9 and cleavage of pro-CASP3, which promotes neuronal survival under pathological conditions.
• Mutations or deletions in NAIP are associated with severe spinal muscular atrophy, as it normally prevents motor neuron apoptosis; it is predominantly expressed in motor neurons and, to a lesser extent, in liver, placenta, and spinal cord.
• In innate immunity, NAIP acts as a specific sensor for bacterial needle protein CprI from Chromobacterium violaceum, triggering NLRC4 inflammasome assembly, caspase-1 activation, cytokine production, and macrophage pyroptosis.
• The protein contains BIR repeat domains, an ATP-binding site, and is regulated by zinc binding, with alternative splicing producing multiple isoforms.
• Keywords: Apoptosis, Inflammatory response, Innate immunity, Metal-binding, Protease inhibitor, Thiol protease inhibitor.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a specific peptide sequence within the C-terminal region (amino acids 750-850) of human NAIP. Confirm that this epitope is preserved in your target isoform or species.
• For Western blotting, a band of approximately 160 kDa is expected; optimize lysis and loading conditions to resolve high-molecular-weight proteins, considering the protein's size.
• In ELISA assays, use the antibody as a capture or detection reagent after validating specificity with recombinant NAIP protein or cell lysates expressing target.
• Given NAIP's role in inflammasome activation, consider stimulating cells with bacterial components before analysis to assess protein expression changes.
• Cross-reactivity with rat has been observed; however, verify reactivity in your model system, as sequence homology may vary across species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves diagnostic manufacturers, laboratories, and research institutes by providing one-stop access to validated IVD raw materials. This NAIP rabbit polyclonal antibody supports research into apoptosis and innate immune pathways. We offer tailored sourcing of rare-target antibodies, recombinant proteins, and ancillary reagents to streamline your assay development.