Anti-NAIP Rabbit Monoclonal Antibody for IHC-P, ELISA - Q13075

Core Product Specifications and Parameters

Biological Background: NAIP Function and Localization

• NAIP (Baculoviral IAP repeat-containing protein 1), also known as neuronal apoptosis inhibitory protein, BIRC1, NLRB1, or psiNAIP, is a member of the inhibitor of apoptosis (IAP) family. It contains three baculoviral IAP repeat (BIR) domains and a nucleotide-binding oligomerization domain (NOD).
• NAIP acts as an anti-apoptotic protein by inhibiting the activities of caspase-3 (CASP3), caspase-7 (CASP7), and caspase-9 (CASP9). It can inhibit the autocleavage of pro-CASP9 and cleavage of pro-CASP3 by CASP9, and it is capable of inhibiting CASP9 autoproteolysis at Asp-315, thus promoting neuronal survival under pathological conditions.
• Mutations or deletions of NAIP are associated with spinal muscular atrophy (SMA), as inappropriate persistence of motor-neuron apoptosis may underlie this disease. NAIP therefore plays a critical role in preventing motor-neuron apoptosis induced by various signals.
• In innate immunity, NAIP functions as a sensor component of the NLRC4 inflammasome. It specifically recognizes and binds needle proteins from pathogenic bacteria such as Chromobacterium violaceum (CprI), leading to inflammasome assembly and activation, caspase-1 activation, cytokine production, and macrophage pyroptosis.
• Expressed predominantly in motor neurons, but not in sensory neurons, NAIP is also found in liver and placenta, and to a lesser extent in the spinal cord.
• NAIP is a multi-functional protein with ATP-binding, metal-binding (zinc), and thiol protease inhibitor activities. It is subject to alternative splicing and is a reference proteome component.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide corresponding to residues 1–100 of human NAIP. This may provide a defined epitope useful for competitive ELISA or peptide blocking experiments.
• For immunohistochemistry (IHC-P), consider using tissues with known NAIP expression such as liver, placenta, or motor neuron-rich regions. Antigen retrieval protocols may be necessary and should be optimized for each tissue type.
• In ELISA applications, the antibody may be suitable for both direct and sandwich formats. A positive control using the immunogen peptide can help verify binding specificity.
• Cross-reactivity with mouse NAIP has been reported, making this antibody a potential tool for studies in mouse models of spinal muscular atrophy or bacterial infection. Validate cross-reactivity in the relevant sample system before quantitative conclusions.
• The protein molecular weight is approximately 160 kDa; ensure appropriate gel percentage and transfer conditions for western blot if attempted (note: not validated for WB).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to providing diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, technical support, and consulting from concept to clinic. This anti-NAIP antibody supports investigations into apoptosis, spinal muscular atrophy, and innate immune inflammasome pathways. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare target raw materials to streamline your IVD development and research projects.