Anti-NAGS Rabbit Polyclonal Antibody - Q8N159

Core Product Specifications and Parameters

Biological Background: N-acetylglutamate synthase (NAGS) Function and Localization

• N-acetylglutamate synthase (NAGS) is a mitochondrial matrix enzyme that catalyzes the formation of N-acetylglutamate (NAG) from glutamate and acetyl-CoA.
• NAG is an essential allosteric cofactor for carbamoylphosphate synthase I (CPS1), the first and rate-limiting enzyme of the urea cycle; thus NAGS plays a critical role in regulating ureagenesis and ammonia detoxification.
• Encoded by the NAGS gene, this protein belongs to the acyltransferase family of enzymes.
• NAGS is highly expressed in adult liver, kidney, and small intestine, with lower expression in fetal tissues and other organs such as lung, pancreas, placenta, heart, and brain.
• The enzyme contains a mitochondrial transit peptide that directs it to the mitochondrial matrix, where it functions.
• Mutations in NAGS are associated with urea cycle disorders (NAGS deficiency), highlighting its importance in nitrogen metabolism; the protein is a target for structural and biochemical studies (3D structure available).
• Keywords associated with NAGS include Acyltransferase, Transferase, Urea cycle, Mitochondrion, and Disease variant.

Experimental Guidance and Technical Tips

• Given the recombinant protein immunogen, the antibody may detect native NAGS in mitochondrial fractions; consider validating in relevant sample systems.
• For Western blot, consider using total cell lysates from human hepatocyte lines or mouse liver tissue, where NAGS is highly expressed at ~58 kDa.
• For ELISA applications, the antibody can be tested as a capture or detection reagent; validate pairing with a suitable partner and assay conditions.
• Since NAGS is mitochondrial, incorporating a mitochondrial fractionation step may improve signal-to-noise ratio in WB or ELISA.
• Cross-reactivity with mouse is reported, so mouse models can be used for in vivo studies; always confirm specificity in your own experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw-material solutions, connecting diagnostic manufacturers with high-quality reagents like this anti-NAGS polyclonal antibody. Whether you need validated antibody pairs for sandwich ELISA, bulk ancillary reagents, or assistance sourcing antibodies against rare metabolic targets, CamelBio's one-stop platform accelerates your assay development from concept to clinic. Leverage our experience in the urea cycle field to streamline your diagnostic kit production.