Anti-NAGA Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P17050

Core Product Specifications and Parameters

Biological Background: Alpha-N-acetylgalactosaminidase (NAGA) Function and Localization

• NAGA encodes alpha-N-acetylgalactosaminidase, also known as alpha-galactosidase B, a lysosomal glycosidase.
• It catalyzes the removal of terminal alpha-N-acetylgalactosamine residues from glycolipids and glycopeptides, essential for glycolipid catabolism.
• The enzyme is localized to the lysosome, where it participates in the degradation of glycoconjugates.
• Post-translational modifications include N-glycosylation (glycoprotein), phosphorylation (phosphoprotein), and the presence of a signal peptide and disulfide bonds, as indicated by UniProt keywords.
• Mutations in NAGA are associated with disease, as noted by the 'Disease variant' keyword, though specific phenotypes may vary; epilepsy is listed among associated keywords.
• The protein has a calculated molecular weight of approximately 46.6 kDa (UniProt), while the antibody product targets a 47 kDa band, consistent with the glycosylated mature form.

Experimental Guidance and Technical Tips

• The immunogen was designed using a recombinant fragment corresponding to amino acids 312–411 near the C-terminus of human NAGA (NP_000253.1). This region may be surface-exposed, potentially enabling recognition of both native and denatured protein.
• For Western blot (WB), a band around 47 kDa is expected; consider using a positive control lysate from tissues or cells known to express NAGA, such as lysosome-rich samples.
• In immunofluorescence/immunocytochemistry (IF/ICC), co-staining with a lysosomal marker is recommended to confirm lysosomal localization.
• The antibody's cross-reactivity with mouse and rat samples suggests conserved epitope recognition; however, sequence alignment is advised to verify epitope conservation if quantitative species comparisons are intended.
• For ELISA applications, optimize coating conditions and use a recombinant NAGA protein or peptide as a standard curve to ensure quantitative accuracy.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with streamlined access to a wide range of IVD raw materials and services, supporting projects from concept to clinic. For lysosomal enzyme research, such as studies on alpha-N-acetylgalactosaminidase (NAGA) in glycolipid metabolism and related disorders, we offer validated antibody pairs, optimized polyclonal antibodies like anti-NAGA, bulk ancillary reagents, and expert sourcing of rare-target raw materials, ensuring your diagnostic development proceeds with reliability and efficiency.