Anti-NAE1 Rabbit Polyclonal Antibody for WB and ELISA - Q13564

Core Product Specifications and Parameters

Biological Background: NAE1 Function and Localization

• NAE1 is the regulatory subunit of the dimeric NEDD8-activating enzyme E1 (UBA3-NAE1), which initiates the neddylation cascade.
• It activates NEDD8 by adenylating its C-terminal glycine with ATP, then forms a thioester-linked NEDD8-UBA3 intermediate before transferring NEDD8 to the E2 enzyme UBE2M.
• The protein is essential for cell cycle progression, particularly at the S-M checkpoint, and its overexpression can trigger apoptosis via deregulated neddylation.
• Neddylation, the covalent attachment of NEDD8 to target proteins, regulates cell growth, viability, and development.
• NAE1 is localized to the cell membrane, and its expression is ubiquitous in fetal tissues and throughout the adult brain.
• Alternative splicing, acetylation, and disease-associated variants (e.g., intellectual disability) have been reported for NAE1.
• The protein has a molecular weight of approximately 60 kDa (UniProt: 60246 Da; observed ~60 kDa).
• Also known as APP-BP1 (amyloid beta precursor protein-binding protein 1), it was originally identified as an interaction partner of the amyloid precursor protein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1-220 amino acids of human NAE1; this region is likely surface-exposed and suitable for generating antibodies that recognize the native protein.
• For Western blot (WB), the expected band size is ~60 kDa; consider using appropriate controls (e.g., lysates from cells with known NAE1 expression) and validate cross-reactivity in mouse and rat samples.
• For ELISA, the polyclonal antibody can be used as a capture or detection reagent; titrate to determine the optimal working concentration.
• Since NAE1 is involved in neddylation, the antibody may be useful for studying protein neddylation dynamics, but confirm specificity with knockdown/overexpression experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research labs, and institutes with streamlined access to high-quality IVD raw materials like this anti-NAE1 antibody, supporting neddylation and cell cycle research. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets to help you accelerate assay development from concept to clinic.