Anti-NADPH oxidase 4 (NOX4) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9NPH5

Core Product Specifications and Parameters

Biological Background: NADPH oxidase 4 Function and Localization

• NADPH oxidase 4 (NOX4) is a member of the NOX family of NADPH oxidases that primarily catalyzes the reduction of oxygen to hydrogen peroxide (H2O2), and to a lesser extent, superoxide, utilizing NADPH as an electron donor. Related references: PMID:14966267 PMID:15356101 PMID:15927447 PMID:10869423 PMID:11032835 PMID:15155719
• NOX4 functions as an oxygen sensor, regulating the KCNK3/TASK-1 potassium channel and HIF1A activity, and is involved in insulin signaling, apoptosis, bone resorption, and lipopolysaccharide-mediated NFKB activation. Related references: PMID:16019190 PMID:14966267 PMID:15356101 PMID:15572675
• It promotes ferroptosis and reactive oxygen species (ROS) production by activating the NLRP3 inflammasome, and modulates redox signaling in vascular cells through ERK1/2 and ELK1 transcription factor activation, potentially inducing nuclear DNA damage. Related references: PMID:39909992 PMID:15721269 PMID:23393389 PMID:16324151
• Widely expressed with highest levels in kidney, and also present in heart, adipocytes, endothelial cells, skeletal muscle, brain, and airway epithelial cells.
• Localizes to multiple subcellular compartments including the endoplasmic reticulum, cell membrane, focal adhesions, cytoplasm, perinuclear region, nucleus, and nucleolus.
• Contains disulfide bonds, is glycosylated, and possesses transmembrane helices; alternative splicing produces isoforms, some of which lack superoxide-generating activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 328-578 of human NOX4; consider this region when designing detection strategies or interpreting epitope-specific results.
• For Western blot (WB), a band at approximately 67 kDa is expected; use appropriate protein ladders and validate in kidney lysates as a positive control.
• In immunohistochemistry (IHC-P), optimize antigen retrieval and antibody dilution; note that NOX4 is strongly expressed in kidney distal tubular cells and endothelial cells.
• The antibody shows cross-reactivity with mouse and rat, making it suitable for translational studies; however, verify performance in your specific experimental system.
• For ELISA, pair with a suitable capture antibody, and consider potential interference from recombinant protein constructs.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, technical services, and consulting, supporting every stage from concept to clinic. This NOX4 rabbit polyclonal antibody exemplifies our capability to provide validated research reagents for oxidative stress, cell signaling, and kidney disease research. Beyond individual antibodies, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and efficient sourcing for rare targets to streamline your diagnostic development workflow.