Anti-NADPH oxidase 4 (NOX4) Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q9NPH5

Core Product Specifications and Parameters

Biological Background: NADPH oxidase 4 (NOX4) Function and Localization

• NADPH oxidase 4 (NOX4), also known as KOX-1 or RENOX, is a member of the NADPH oxidase family that primarily catalyzes the reduction of oxygen to hydrogen peroxide (H2O2), and to a lesser extent superoxide (PubMed:14966267, PubMed:15356101, PubMed:15927447, PubMed:10869423, PubMed:11032835, PubMed:15155719). Related references: PMID:14966267, PMID:15356101, PMID:15927447, PMID:10869423, PMID:11032835, PMID:15155719
• It functions as an oxygen sensor, regulating the KCNK3/TASK-1 potassium channel and HIF1A activity (PubMed:16019190). Related references: PMID:16019190
• NOX4 participates in insulin signaling, apoptosis, bone resorption, and lipopolysaccharide-mediated NF-kappa-B activation (PubMed:14966267, PubMed:15356101, PubMed:15572675). Related references: PMID:14966267, PMID:15356101, PMID:15572675
• The enzyme contributes to redox signaling in vascular cells by modulating ERK1/2 and ELK1 transcription factor activation and inducing nuclear DNA damage (PubMed:23393389). Related references: PMID:23393389
• NOX4 promotes ferroptosis and ROS production via NLRP3 inflammasome activation and cytokine release (PubMed:39909992). Related references: PMID:39909992
• It localizes to multiple subcellular compartments including the cytoplasm, endoplasmic reticulum membrane, cell membrane, focal adhesions, nucleus, nucleolus, and perinuclear region.
• Widely expressed, with highest levels in the kidney; also present in heart, adipose tissue, endothelial cells, skeletal muscle, brain, and airway epithelial cells.
• Post-translationally, NOX4 is a glycoprotein with disulfide bonds and transmembrane domains, and undergoes alternative splicing.

Experimental Guidance and Technical Tips

• The recombinant protein immunogen is designed against human NOX4; validate specificity in the relevant species and cell/tissue type.
• For Western blotting, anticipate a band around 67 kDa; be aware of potential post-translational modifications that may shift migration.
• In immunofluorescence/ICC, NOX4 exhibits multiple localizations; appropriate fixation and permeabilization may be required to detect membrane, cytoplasmic, or nuclear pools.
• ELISA performance may depend on the antibody pair and antigen format; consider using recombinant NOX4 protein as a standard.
• Human cross-reactivity is indicated; verify in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive one-stop access to IVD raw materials, technical services, and consulting—supporting your journey from concept to clinic. This high-quality anti-NOX4 rabbit monoclonal antibody is part of our extensive portfolio for oxidative stress and redox biology research. Beyond individual antibodies, we supply validated antibody pairs, optimized monoclonals, bulk ancillary reagents, and specialize in sourcing rare-target raw materials to meet your diagnostic development needs.