Anti-NADPH oxidase 4 (NOX4) Polyclonal Antibody for WB, ELISA - Q9JHI8

Core Product Specifications and Parameters

Biological Background: NADPH oxidase 4 (NOX4) Function and Localization

• NADPH oxidase 4 (NOX4) is a multi-pass membrane protein (UniProt keywords: Transmembrane, Transmembrane helix) belonging to the NADPH oxidase family. It is also known as Kidney oxidase-1 (KOX-1), Renal NAD(P)H-oxidase (RENOX), and Superoxide-generating NADPH oxidase 4.
• Primarily catalyzes the reduction of oxygen to hydrogen peroxide (H2O2) (By similarity), and to a lesser extent, superoxide (PubMed:10869423, PubMed:11098048, PubMed:15638999). Related references: PMID:10869423, PMID:11098048, PMID:15638999
• Functions as an oxygen sensor regulating potassium channels (e.g., KCNK3/TASK-1) and HIF1A activity, and modulates insulin signaling, apoptosis, bone resorption, and NF-kB activation (By similarity).
• Promotes ferroptosis and reactive oxygen species (ROS) production by activating the NLRP3 inflammasome, leading to cytokine release and reduced glutathione levels (By similarity).
• Subcellular localization includes cytoplasm, endoplasmic reticulum membrane, cell membrane, focal adhesions, nucleus, and nucleolus (UniProt).
• Expressed in multiple tissues: brain (cerebellum, Ammon horn, Purkinje cells), osteoclasts, leukocytes, kidney, liver, lung, and vascular smooth muscle cells (UniProt).
• Post-translational modifications include glycosylation and disulfide bond formation (UniProt keywords).

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight is approximately 67 kDa; validate in relevant mouse or rat tissue/cell lysates, considering that NOX4 is a transmembrane protein that may require optimized solubilization conditions.
• The immunogen is a recombinant fusion protein covering amino acids 307-424; the antibody may recognize multiple isoforms or post-translationally modified forms, so additional confirmation by specific controls is recommended.
• For ELISA, consider using the recombinant immunogen as a positive control and titrate the antibody against target-coated plates to determine optimal working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and researchers with streamlined access to IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this NOX4-targeting reagent for redox biology and oxidative stress studies. Our one-stop sourcing capabilities extend to bulk ancillary reagents, rare target antibodies, and comprehensive technical support, all designed to accelerate your development pipeline. Partner with CamelBio to bridge the gap between concept and clinic with reliable, high-quality raw materials.