Anti-NACA Rabbit Monoclonal Antibody for WB, ELISA - Q13765

Core Product Specifications and Parameters

Biological Background: NACA Function and Localization

• NACA encodes nascent polypeptide-associated complex subunit alpha (Alpha-NAC), a 23.4 kDa protein with multiple isoforms generated by alternative splicing.
• Serves as a chaperone that prevents inappropriate targeting of non-secretory nascent polypeptide chains to the endoplasmic reticulum by blocking interaction with the signal recognition particle (SRP).
• Also reduces ribosome affinity for protein translocation sites in the ER membrane, ensuring fidelity of co‑translational trafficking.
• Acts as a transcriptional coactivator: binds DNA and stabilizes JUN homodimer interaction with target gene promoters, linking translation to transcription regulation.
• Localizes to both the cytoplasm and nucleus, consistent with its dual roles in protein biogenesis and gene expression.
• Ubiquitously expressed across human tissues, indicating a housekeeping function.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination; additionally annotated as an allergen and a DNA-binding protein.

Experimental Guidance and Technical Tips

• The immunogen (residues 1‑215) covers the N‑terminal region of NACA; consider epitope conservation when testing cross‑reactivity in non‑human samples.
• For Western blotting, NACA typically migrates around 23 kDa; validate the observed band size with appropriate molecular weight markers and loading controls.
• ELISA applications may benefit from optimizing coating antigen concentration and antibody dilution based on the assay format; cross‑reactivity with related NAC subunits should be assessed experimentally.
• Because NACA shuttles between cytoplasm and nucleus, sample preparation (e.g., subcellular fractionation) could be considered to enrich the target in specific compartments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with seamless access to IVD raw materials, ancillary reagents, and technical consulting. This Anti‑NACA antibody supports investigations into co‑translational translocation and transcriptional regulation. Beyond this product, our supply portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare‑target raw‑material sourcing—covering every stage from concept to clinic.