Anti-NAB2 Rabbit Polyclonal Antibody for WB, ELISA - Q15742

Core Product Specifications and Parameters

Biological Background: NAB2 Function and Localization

• NAB2 (NGFI-A-binding protein 2), also known as EGR-1-binding protein 2 and MADER, is a transcriptional repressor that interacts with zinc finger transcription factors EGR1 and EGR2.
• The protein functions as a corepressor to inhibit transcription mediated by EGR1 and EGR2, regulating gene expression involved in cellular processes.
• Alternative splicing generates multiple isoforms; isoform 2 lacks repression ability, suggesting functional regulation at the isoform level.
• NAB2 is localized predominantly in the nucleus, consistent with its role in transcriptional regulation.
• It is widely expressed at low levels in various tissues, with notably high expression in melanoma cell lines, implicating it in melanoma biology.
• Post-translational modifications include phosphorylation and ubiquitination (isopeptide bond), which may modulate its activity or stability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-200) of human NAB2, which is conserved in the canonical isoform; consider verifying isoform specificity in your experimental system.
• For western blot (WB), the predicted molecular weight of NAB2 is approximately 57 kDa; use appropriate protein markers and validate with positive controls.
• This antibody cross-reacts with both human and mouse NAB2, making it suitable for comparative studies; however, confirm cross-reactivity in your specific sample type.
• ELISA application may require optimization of coating antigen and antibody titration; consider using recombinant protein as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw material procurement, supporting research and diagnostic development from concept to clinic. This anti-NAB2 polyclonal antibody is part of our extensive catalog of high-quality antibodies tailored for transcription regulation studies. Beyond individual antibodies, we offer validated antibody pairs, optimized monoclonals, bulk ancillary reagents, and sourcing of rare-target raw materials to streamline your workflow.