Anti-NAB1 Polyclonal Antibody for WB, ELISA - Q13506

Core Product Specifications and Parameters

Biological Background: NAB1 Function and Localization

• NAB1 (NGFI-A-binding protein 1) is also known as EGR-1-binding protein 1 or transcriptional regulatory protein p54. It acts as a transcriptional repressor for the zinc finger transcription factors EGR1 and EGR2.
• NAB1 is localized predominantly in the nucleus, consistent with its role in transcriptional regulation.
• Alternative splicing generates multiple isoforms, including a short isoform found in myeloid leukemia cell lines (e.g., KG-1).
• Post-translational modifications include phosphorylation and ubiquitin-like conjugation (isopeptide bond), which may regulate its activity or stability.
• Part of the NAB family of transcriptional corepressors, which modulate immediate-early gene responses.
• Keywords: 3D-structure, Alternative splicing, Isopeptide bond, Nucleus, Phosphoprotein, Proteomics identification, Reference proteome, Repressor, Transcription, Transcription regulation, Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 189-317) of human NAB1. This region is conserved across species, consistent with the observed cross-reactivity with mouse.
• For Western blot, a band around 54 kDa is expected; validate detection in the relevant sample systems.
• For ELISA, the antibody may be used in direct or sandwich formats; consider optimizing dilution and detection conditions in your assay.
• As a transcriptional repressor, NAB1 expression levels may vary by cell type and stimulation; include appropriate controls in experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with validated raw materials for transcriptional regulation targets like NAB1. We supply optimized polyclonal antibodies, bulk ancillary reagents, and can source rare-target raw materials to meet your IVD development needs. Let CamelBio streamline your procurement from discovery to clinical application.