Anti-NAA50 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9GZZ1

Core Product Specifications and Parameters

Biological Background: NAA50 Function and Localization

• NAA50 (N-alpha-acetyltransferase 50) is also known as NAT13, NAT5, hSAN, and is the catalytic subunit of the NatE complex.
• Functions as an N-alpha-acetyltransferase, acetylating the N-terminus of proteins that retain their initiating methionine; displays broad substrate specificity except when proline is in the second position. Related references: PMID:19744929 PMID:21900231 PMID:22311970 PMID:27484799
• Additionally exhibits N-epsilon-acetyltransferase activity, mediating acetylation of specific lysine side chains, and undergoes autoacetylation; however, the in vivo relevance of epsilon-acetylation remains unclear. Related references: PMID:19744929
• Component of N-terminal acetyltransferase complexes with NAA10 and NAA15; while it does not alter NAA10 activity directly, it negatively regulates the NatA complex’s overall acetyltransferase function. Related references: PMID:16507339 PMID:27484799 PMID:29754825 PMID:32042062
• Essential for sister chromatid cohesion during mitosis by promoting the binding of CDCA5/sororin to cohesin, likely through counteracting NAA10 activity. Related references: PMID:17502424 PMID:27422821
• Predominantly localized in the cytoplasm and nucleus.
• The protein has a theoretical molecular weight of approximately 19.4 kDa (UniProt Q9GZZ1), consistent with the observed ~19 kDa band.
• Keywords indicate it is an acetyltransferase, subject to alternative splicing and phosphorylation, and identified in proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-169) of human NAA50, suggesting potential reactivity with full-length protein. Consider validating antibody specificity in your target species and experimental system.
• This antibody is validated for WB, IHC-P, IF/ICC, and ELISA. For WB, a band around 19 kDa is expected. Optimize antibody dilution and detection conditions for your specific sample type.
• Demonstrated cross-reactivity with human, mouse, and rat. If using with other species, verify performance prior to experiments.
• As a polyclonal antibody, lot-to-lot variability may occur. Consistent lot usage is recommended for longitudinal studies to ensure reproducibility.
• If high background is observed, consider reducing antibody concentration and increasing blocking stringency.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with integrated access to IVD raw materials, including validated NAA50 antibodies for studying protein acetylation and cohesion pathways. Our portfolio covers bulk antibodies, recombinant proteins, and tailored sourcing for rare targets, supporting your assay development from concept to clinic.