Anti-NAA50 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9GZZ1

Core Product Specifications and Parameters

Biological Background: NAA50 Function and Localization

• NAA50 (N-alpha-acetyltransferase 50), also known as NAT13, NAT5, SAN, MAK3, and hNaa50p, is an acetyltransferase family member that undergoes alternative splicing.
• It catalyzes N-terminal acetylation of proteins retaining their initiator methionine, showing broad substrate specificity but unable to acetylate peptides with proline in the second position. Related references: PMID:19744929, PMID:21900231, PMID:22311970, PMID:27484799
• NAA50 also displays N-epsilon-acetyltransferase activity, acetylating specific lysine residues (such as histone H4 in vitro) and undergoing autoacetylation in vivo. Related references: PMID:19744929
• It is a component of N-alpha-acetyltransferase complexes with NAA10 and NAA15, where it negatively regulates the NatA complex N-alpha-acetyltransferase activity. Related references: PMID:16507339, PMID:27484799, PMID:29754825, PMID:32042062
• NAA50 is essential for sister chromatid cohesion during mitosis by facilitating CDCA5/sororin binding to cohesin, likely counteracting NAA10 function. Related references: PMID:17502424, PMID:27422821
• The protein localizes to both cytoplasm and nucleus, consistent with its roles in translation-coupled acetylation and chromatin dynamics.
• Post-translational modifications identified by proteomics include acetylation and phosphorylation, contributing to its regulation.

Experimental Guidance and Technical Tips

• For Western blot (WB), expect a band at approximately 19 kDa; use positive controls from human, mouse, or rat cell lines (e.g., HeLa, HEK293, NIH/3T3).
• In immunofluorescence/immunocytochemistry (IF/ICC), optimize fixation and permeabilization; NAA50 signal may be observed in both cytoplasmic and nuclear compartments.
• For ELISA applications, the recombinant immunogen (aa 1–169) can serve as a positive control; note that polyclonal antibodies may exhibit lot-to-lot variability.
• Cross-reactivity with human, mouse, and rat has been reported; verify sequence homology before using on other species.
• Consult public expression databases (e.g., Human Protein Atlas) to select tissues or cell lines with adequate NAA50 expression for your experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with a comprehensive, one-stop supply of IVD raw materials. Our NAA50 rabbit polyclonal antibody supports studies on protein acetylation, epigenetic regulation, and chromosome dynamics. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to bridge your development from concept to clinic.