Anti-NAA25 Polyclonal Antibody for WB and ELISA - Q14CX7

Core Product Specifications and Parameters

Biological Background: NAA25 Function and Localization

• NAA25, also known as NAP1, MDM20, or C12orf30, is the non-catalytic auxiliary subunit of the N-terminal acetyltransferase B (NatB) complex.
• The NatB complex catalyzes the acetylation of the N-terminal methionine of nascent peptides beginning with Met-Asp, Met-Glu, Met-Asn, or Met-Gln.
• NAA25 may contribute to normal cell-cycle progression through its role in protein N-terminal acetylation.
• The protein localizes predominantly to the cytoplasm.
• NAA25 contains TPR repeat motifs, is subject to alternative splicing, and its 3D structure has been partially resolved.
• The full-length protein has a calculated molecular weight of approximately 112 kDa.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 703-972 of human NAA25, a region likely exposed in the full-length protein; consider this when evaluating isoform reactivity.
• For Western blot, a band near 112 kDa is expected in human, mouse, and rat lysates; optimize loading and transfer conditions for high-molecular-weight proteins.
• In ELISA, use the recombinant protein or peptide coating; validate specificity against the target sequence.
• As a polyclonal antibody, lot-to-lot consistency should be verified in the relevant sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers with integrated access to IVD raw materials, from single antibodies to complete reagent sets. For NAA25-related studies—spanning protein acetylation, cell-cycle control, and post-translational modification—we can supply validated antibody pairs, monoclonal/polyclonal options, and ancillary bulk reagents. Our sourcing network also handles rare-target raw materials, ensuring a streamlined path from discovery to clinical assay development.