Anti-NAA20 Rabbit Polyclonal Antibody for WB, IHC-P, IP, ELISA - P61599

Core Product Specifications and Parameters

Biological Background: NAA20 Function and Localization

• NAA20 (also known as NAT5, NAT3, NatB catalytic subunit) is the catalytic subunit of the NatB N-terminal acetyltransferase complex, responsible for the acetylation of the N-terminal methionine of nascent proteins.
• The NatB complex specifically acetylates peptides with N-terminal sequences Met-Asp, Met-Glu, Met-Asn, or Met-Gln, as demonstrated by functional studies. Related references: PMID:34230638
• Proteins involved in cell cycle regulation are notably enriched among NatB substrates, highlighting a critical role in cell division.
• NAA20 is essential for maintaining actomyosin fiber structure and function, as well as supporting proper cellular migration.
• Subcellularly, NAA20 localizes to both the cytoplasm and the nucleus, consistent with its broad regulatory roles.
• Alternative splicing of NAA20 generates multiple isoforms, and mutations in the gene are associated with autosomal recessive intellectual disability (MRT73).
• Additional keywords include Acyltransferase and Transferase activities, with evidence from proteomics identification and 3D-structure studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 49-178 of human NAA20, a region potentially conserved in mouse and rat; cross-reactivity should be validated in the relevant species.
• In Western blotting, a band near 20 kDa is expected; optimize antibody dilution and use appropriate loading controls.
• For IHC-P, consider antigen retrieval and titration experiments, as NAA20 can be detected in both cytoplasmic and nuclear compartments.
• When performing immunoprecipitation, the antibody may co-immunoprecipitate other subunits of the NatB complex; confirm with appropriate controls.
• ELISA applications can utilize recombinant NAA20 protein or peptide antigens for validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutions with comprehensive IVD raw material sourcing, technical services, and consulting. For NAA20-related studies and assay development, CamelBio offers validated polyclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement, streamlining the path from concept to clinical application.