Anti-NAA10 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P41227

Core Product Specifications and Parameters

Biological Background: NAA10 Function and Localization

• Protein Identity and Complex: NAA10 (ARD1) is the catalytic subunit of the NatA N-terminal acetyltransferase complex, which co-translationally acetylates the N-termini of nascent proteins. Related references: PMID:15496142, PMID:19420222, PMID:19826488

• Epsilon Acetyltransferase Activity: In the absence of its auxiliary subunit NAA15, NAA10 can display epsilon (internal lysine) acetyltransferase activity, targeting substrates such as HIF1A for degradation. Related references: PMID:12464182

• Tumor Suppressive Roles: NAA10 acetylates MYLK to repress tumor cell migration, and acetylates and stabilizes TSC2 to inhibit mTOR signaling, thereby suppressing cancer development. Related references: PMID:19826488, PMID:20145209

• Chaperone Regulation: NAA10 acetylates HSPA1A and HSPA1B at Lys-77, enhancing chaperone activity and promoting interaction with the co-chaperone HOPX. Related references: PMID:27708256

• Histone Modification: Acetylates histone H4 (HIST1H4A), contributing to epigenetic regulation. Related references: PMID:29754825

• Mitotic Regulation: Functions as a negative regulator of sister chromatid cohesion during mitosis. Related references: PMID:27422821

• Subcellular Localization: Localizes to both the cytoplasm and nucleus.

• Tissue Expression: Ubiquitously expressed across tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 161-235) of human NAA10. When detecting specific isoforms or mutants, consider sequence alignment to confirm epitope conservation.
• For Western blot, the predicted molecular weight is approximately 26 kDa. Use appropriate positive and negative control lysates (e.g., human, mouse, rat cell lines) and validate band specificity.
• In IHC-P and IF/ICC, optimize antigen retrieval and fixation/permeabilization conditions. Include isotype controls and tissue/cell samples with known NAA10 expression levels.
• For ELISA applications, titrate antibody concentration and consider pairing with a matched detection antibody if developing sandwich assays.
• As a polyclonal antibody, lot-to-lot variability may occur; we recommend confirming reactivity in your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research teams a consolidated source for IVD raw materials, combining this high-quality anti-NAA10 antibody with a broad portfolio of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and tailored sourcing for rare targets. Whether you are developing acetylation-related or cancer biomarker platforms, our supply capabilities help streamline your workflow from concept to clinic.