Anti-N6AMT1/HEMK2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9Y5N5

Core Product Specifications and Parameters

Biological Background: N6AMT1/HEMK2 Function and Localization

• N6AMT1, also known as HEMK2, KMT9, or PrmC, is a methyltransferase that catalyzes methylation of both proteins and, to a lesser extent, arsenic. Related references: PMID:18539146, PMID:21193388, PMID:30017583
• It is the catalytic subunit of a heterodimer with TRMT112, which monomethylates 'Lys-12' of histone H4 (H4K12me1), a mark found at promoters of cell cycle regulator genes. Related references: PMID:31061526
• The enzyme also catalyzes N5-methylation of glutamine residues on proteins containing a Gly-Gln-Xaa-Xaa-Xaa-Arg motif, including the translation termination factor ETF1 on 'Gln-185'. Related references: PMID:18539146, PMID:31632689, PMID:31636962
• In arsenic metabolism, N6AMT1 may mediate the conversion of toxic monomethylarsonous acid into less toxic dimethylarsonic acid, though its role is limited compared to AS3MT. Related references: PMID:21193388, PMID:25997655
• Subcellular localization is primarily nuclear, consistent with its role in histone modification and chromatin regulation.
• It is widely expressed, with highest levels in parathyroid and pituitary glands, followed by adrenal gland and kidney, and lowest in leukocytes and mammary gland.
• Keywords: 3D-structure, Alternative splicing, Chromatin regulator, Methyltransferase, Nucleus, Proteomics identification, Reference proteome, S-adenosyl-L-methionine, Transferase, Ubl conjugation.

Experimental Guidance and Technical Tips

• Immunogen: Recombinant fusion protein covering amino acids 1-214 of human N6AMT1/HEMK2. This full-length immunogen suggests broad epitope recognition, but consider potential cross-reactivity with other methyltransferases sharing structural homology; validate specificity using knockout or knockdown controls.
• For Western blot (WB), endogenous N6AMT1/HEMK2 is predicted at ~23 kDa. Ensure adequate separation by SDS-PAGE and optimize primary antibody dilution for your sample type and detection system.
• For immunohistochemistry (IHC-P), tissues with high expression (e.g., pituitary, adrenal gland) may serve as positive controls, while leukocytes or mammary gland may show weaker staining. Include appropriate antigen retrieval and normal IgG controls.
• ELISA applications may be used for quantitative detection; consider pairing with a detection antibody and optimizing the capture/detection format for your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For chromatin biology and arsenic toxicity research, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development. Whether you need specialized reagents for N6AMT1/HEMK2 or comprehensive support for your diagnostic pipeline, CamelBio bridges the gap between concept and clinical application.