Anti-N4BP1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O75113

Core Product Specifications and Parameters

Biological Background: N4BP1 Function and Localization

• N4BP1 (NEDD4-binding protein 1) is a potent suppressor of cytokine production and a key negative regulator of innate immune signaling, limiting inflammatory responses to minor TLR stimuli.
• Acts as a restriction factor against HIV-1 by binding viral mRNAs and degrading them through its intrinsic ribonuclease activity. Related references: PMID:31133753
• Upon infection with more threatening pathogens, N4BP1 is cleaved by CASP8 downstream of TLR3 or TLR4, leading to its inactivation and allowing full cytokine production.
• Inhibits the E3 ubiquitin-protein ligase ITCH by competing for substrate binding via the second WW domain of ITCH, impairing ubiquitination.
• Localizes to the cytoplasm, cytosol, nucleus, nucleolus, and PML nuclear bodies, indicating multifunctional cellular distribution.
• Detected in a wide range of tissues, including heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis, and ovary.
• Keywords from UniProt highlight its roles as a hydrolase, nuclease, RNA-binding protein, and phosphoprotein involved in innate immunity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 190–590 of mouse N4BP1, a region containing the NYN (RNase) domain and part of the ITCH-interacting region. This design is expected to produce an antibody that recognizes full-length N4BP1 in denaturing and native conditions.
• For Western blot, consider a starting dilution range of 1:500–1:2000 and validate with relevant positive controls (e.g., human or mouse cell lysates known to express N4BP1).
• In immunofluorescence/ICC, confirm nuclear and cytoplasmic localization with appropriate fixation and permeabilization protocols; inclusion of PML body markers may aid co-localization studies.
• The antibody is validated for ELISA, potentially suitable for capture or detection in sandwich assays; optimize coating and detection conditions for your specific immunoassay format.
• Cross-reactivity with human and mouse N4BP1 is specified; however, verify specificity in your experimental system, especially if working with samples from other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research teams with comprehensive access to IVD raw materials, including validated antibody pairs, bulk ancillary reagents, and rare target sourcing. This N4BP1 rabbit polyclonal antibody supports innate immunity and viral restriction research, complementing our portfolio of immune signaling reagents. Explore our one-stop sourcing services to accelerate your assay development from concept to clinic.