Anti-N-WASP/WASL Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O00401

Core Product Specifications and Parameters

Biological Background: N-WASP/WASL Function and Localization

• WASL/N-WASP stimulates actin nucleation via the Arp2/3 complex, serving as a central regulator of cytoskeletal dynamics. Related references: PMID:16767080, PMID:19366662, PMID:19487689
• It is essential for mitosis and cytokinesis through actin polymerization. Related references: PMID:19366662, PMID:19487689, PMID:22847007
• N-WASP cooperates with CDC42 to promote filopodia formation, extending thin actin-rich surface projections. Related references: PMID:9422512
• In the nucleus, it regulates gene transcription by associating with HSF1 on heat shock promoter elements (By similarity).
• Participates in dendrite spine morphogenesis (By similarity) and maintains apical junction linearity in epithelial cells. Related references: PMID:19767742
• Localizes to the cytoplasm, cytoskeleton, and nucleus, reflecting its multifunctional roles.
• Post‑translationally modified by phosphorylation, acetylation, and methylation.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight is ~55 kDa; use appropriate positive controls (e.g., HeLa or Jurkat lysates) and consider blocking with 5% non‑fat milk.
• In IHC‑P, validate antigen retrieval methods (e.g., heat‑induced citrate buffer, pH 6.0) and include isotype controls to assess specificity.
• For IF/ICC, fixation and permeabilization conditions may influence staining; test paraformaldehyde versus methanol fixation and optimize antibody dilution.
• The antibody is expected to detect N-WASP in human, mouse, and rat samples; cross‑reactivity with other species has not been tested.

CamelBio: Your One-Stop Sourcing Bridge

As a trusted IVD raw material partner, CamelBio supplies high-quality antibodies like this N-WASP/WASL polyclonal antibody to advance cytoskeleton and cell signaling research. We offer comprehensive sourcing solutions, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, bridging your development from concept to clinic. For rare or specialized targets, our team ensures reliable access to critical raw materials, supporting your diagnostic manufacturing needs.