Anti-N-WASP/WASL Monoclonal Antibody for WB, ELISA - O00401

Core Product Specifications and Parameters

Biological Background: N-WASP/WASL Function and Localization

• N-WASP/WASL, also known as Neural Wiskott-Aldrich syndrome protein, is an actin nucleation-promoting factor encoded by the WASL gene in humans.
• It stimulates actin polymerization by activating the Arp2/3 complex, playing a crucial role in cytoskeletal reorganization (PubMed:16767080, PubMed:19366662, PubMed:19487689). Related references: PMID:16767080, PMID:19366662, PMID:19487689
• Involved in mitosis and cytokinesis through regulation of actin dynamics (PubMed:19366662, PubMed:19487689, PubMed:22847007). Related references: PMID:19366662, PMID:19487689, PMID:22847007
• Cooperates with CDC42 to extend and maintain filopodia, thin actin-rich surface projections (PubMed:9422512). Related references: PMID:9422512
• Functions in the nucleus by promoting nuclear actin polymerization and regulating gene transcription, including negative regulation of HSP90 expression via HSF1 binding (By similarity).
• Plays a role in dendrite spine morphogenesis (By similarity).
• Decreasing levels of DNMBP alters apical junction morphology in enterocytes, indicating a role in cell junction integrity (PubMed:19767742). Related references: PMID:19767742
• Localizes to the cytoplasm, cytoskeleton, and nucleus; post-translational modifications include acetylation, phosphorylation, and methylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide from amino acids 350-450 of human N-WASP/WASL. This region is unique and likely to generate specific reactivity.
• For Western blotting, use whole cell lysates from human, mouse, or rat cell lines; expect a band at approximately 55kDa. Include appropriate positive and negative controls to ensure specificity.
• In ELISA applications, this antibody may be used as a capture or detection component in sandwich assays; validate pair compatibility with recombinant protein or cell-derived samples.
• When working with mouse or rat samples, consider verifying cross-reactivity experimentally due to potential species-specific differences.
• Optimal working dilutions should be determined empirically for each experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw material sourcing, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target procurement. This anti-N-WASP/WASL antibody supports cytoskeletal and cell motility research and diagnostic development. From concept to clinic, we provide the materials and technical support to accelerate your projects.