Anti-N-Cadherin Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P19022

Core Product Specifications and Parameters

Biological Background: N-Cadherin Function and Localization

• Cadherin-2 (N-cadherin) is a calcium-dependent cell adhesion protein that preferentially mediates homotypic cell-cell adhesion by dimerization, contributing to the sorting of heterogeneous cell types.
• It acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone; cleavage by MMP24 modulates this quiescence.
• CDH2 is required for proper neurite branching and pre- and postsynaptic organization, and may regulate dendritic spine density in hippocampal neurons.
• The protein localizes to the cell membrane, sarcolemma, adherens junctions, desmosomes, and axon projections, indicating diverse roles in cell adhesion and signaling.
• Post-translational modifications include cleavage on pair of basic residues, and it is a glycoprotein and phosphoprotein.
• According to UniProt keywords, CDH2 is associated with disease variants including cardiomyopathy and intellectual disability, and features multiple repeat domains and a signal peptide.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the cytoplasmic domain of N-Cadherin (aa 807-906), a region conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For western blotting, the predicted molecular weight is approximately 100 kDa; consider using appropriate molecular weight markers and optimizing transfer conditions for high-molecular-weight proteins.
• In immunohistochemistry (IHC-P), antigen retrieval and antibody titration are recommended; validation in relevant tissue types (e.g., neural, cardiac, or tumor samples) is advised.
• For ELISA applications, consider pairing this antibody with suitable capture or detection reagents and validate assay sensitivity in your specific matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This N-Cadherin polyclonal antibody supports studies in neurobiology, oncology, and cardiovascular disease. CamelBio can assist with sourcing validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw materials to accelerate your diagnostic development.