Anti-N-Cadherin Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P19022

Core Product Specifications and Parameters

Biological Background: N-Cadherin Function and Localization

• Cadherin-2 (N-Cadherin, CDw325) is a calcium-dependent cell adhesion protein that preferentially mediates homotypic cell-cell adhesion via dimerization with CDH2 chains from adjacent cells, contributing to the sorting of heterogeneous cell types.
• It acts as a regulator of neural stem cell quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone; cleavage by MMP24 affects this anchorage, modulating quiescence.
• CDH2 plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting process formation by NCS cells.
• Required for proper neurite branching and pre- and postsynaptic organization; may regulate dendritic spine density in hippocampal neurons.
• May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips.
• Subcellularly localized to the cell membrane, sarcolemma, cell junctions (adherens junctions, desmosomes), cell surface, and axon projections.
• Classified under keywords including alternative splicing, calcium binding, cardiomyopathy, cell adhesion, glycoprotein, disease variant, intellectual disability, metal-binding, phosphoprotein, and transmembrane helix.
• Molecular weight of the canonical isoform is approximately 99.8 kDa (UniProt), consistent with the observed 100 kDa band.

Experimental Guidance and Technical Tips

• For Western blot, the antibody detects a band around 100 kDa (corresponding to full-length N-Cadherin). Ensure reducing conditions and use appropriate positive controls such as human, mouse, or rat brain or heart lysates.
• In immunofluorescence (IF-P) and immunohistochemistry (IHC-P), membrane localization is expected; consider optimizing fixation and permeabilization to preserve epitope accessibility.
• For ELISA, the antibody may be used as a capture or detection reagent; validate with known standards.
• Note that the immunogen corresponds to an extracellular domain region (aa 488-587), which is conserved across human, mouse, and rat, consistent with the observed cross-reactivity.
• Endogenous N-Cadherin may be expressed at varying levels in different tissues; include appropriate loading controls and validate species-specific expression.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, from validated antibody pairs and optimized polyclonal antibodies like this N-Cadherin antibody, to bulk ancillary reagents and rare target sourcing. Our supply capabilities support neuroscience and cell adhesion research, ensuring seamless transition from concept to clinic. For customized sourcing or technical consultation, rely on CamelBio as your dedicated partner.