Anti-MZT2B Polyclonal Antibody for WB, ELISA - Q6NZ67

Core Product Specifications and Parameters

Biological Background: MZT2B Function and Localization

• MZT2B (Mitotic-spindle organizing protein 2B), also known as FAM128B or MOZART2B, is a centrosome-associated protein essential for mitotic spindle formation.
• It facilitates the recruitment and assembly of the gamma-tubulin ring complex (gTuRC) at the centrosome, a critical step for minus-end nucleation of microtubules and proper spindle assembly. Related references: PMID:20360068 PMID:39321809
• The protein localizes predominantly to the cytoplasm, specifically at the centrosome and mitotic spindle, consistent with its role in microtubule organization.
• MZT2B is a phosphoprotein, indicating regulation by phosphorylation during the cell cycle, and its molecular weight is predicted at 16226 Da, migrating at approximately 16 kDa in SDS-PAGE.
• Structural studies have resolved its 3D structure, and it has been identified in multiple proteomics screens as a component of the centrosome.

Experimental Guidance and Technical Tips

• Since the immunogen covers the full-length protein (aa 1-158), the polyclonal antibody may recognize multiple epitopes, offering robust detection in denaturing western blotting and native ELISA.
• For WB, consider using whole-cell lysates from actively dividing human cell lines (e.g., HeLa, HEK293T) to enrich for mitotic spindle proteins. Optimize primary antibody dilution through a range-finding experiment, and include appropriate controls to verify specificity.
• The antibody is reactive with human samples only; cross-reactivity with other species has not been reported, so validate in the relevant human model system.
• For ELISA, the antibody can be used as a capture or detection reagent, but assay conditions should be optimized for the specific format (direct, indirect, or sandwich).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to high-quality IVD raw materials like this anti-MZT2B antibody, supporting centrosome and cell cycle research applications. Beyond individual antibodies, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw materials — bridging the gap from early-stage discovery to clinical application. Let us be your reliable sourcing partner, ensuring consistent supply and technical support for your next project development.