Anti-MZF1 Polyclonal Antibody for WB, ELISA - P28698

Core Product Specifications and Parameters

Biological Background: Myeloid zinc finger 1 Function and Localization

• Myeloid zinc finger 1 (MZF1), also known as zinc finger and SCAN domain-containing protein 6 (ZSCAN6) and zinc finger protein 42 (ZNF42), belongs to the krüppel C2H2-type zinc-finger protein family.
• Functions as a transcription regulator by binding to specific promoter DNA sequences. It regulates the expression of key target genes including PADI1 and CDH2 (N-cadherin), potentially influencing cellular processes such as differentiation and adhesion.
• Contains an N-terminal SCAN domain, which mediates protein-protein interactions and homodimerization, and a C-terminal array of zinc finger motifs responsible for DNA binding.
• Primarily localizes to the nucleus, consistent with its role as a transcription factor.
• Preferentially expressed in differentiating myeloid cells and also detected in osteoblasts, suggesting a role in hematopoietic and bone development.
• Subject to post-translational modifications including phosphorylation and ubiquitination, which may modulate its activity, stability, or interactions.
• Alternative splicing generates multiple isoforms, potentially providing functional diversity.
• Contains multiple zinc finger domains of the C2H2 type, which facilitate sequence-specific DNA binding.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 100–350, a central region of MZF1. Confirm sequence identity with relevant species to predict cross-reactivity.
• As a polyclonal antibody, it may detect multiple MZF1 isoforms; consider this when interpreting blotting patterns.
• For Western blot, validate in myeloid lineage cells (e.g., HL-60, U937) that endogenously express MZF1, using appropriate nuclear extraction protocols.
• In ELISA, use purified recombinant protein or cell lysates from expressing cells; optimize coating concentrations and detection conditions.
• Verify antibody specificity by knocking down MZF1 expression or using peptide competition assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement by offering validated antibodies, optimized monoclonal/polyclonal antibody pairs, and bulk ancillary reagents, supporting projects from transcription factor research to hematological assay development. For investigators targeting MZF1 in myeloid differentiation or gene regulation, we provide reliable sourcing of this polyclonal antibody alongside comprehensive technical consultation. Rely on CamelBio as your one-stop bridge from concept to clinic, with efficient access to rare-target raw materials and flexible supply solutions.