Anti-MZB1 Polyclonal Antibody for WB, IHC-P, ELISA - Q8WU39

Core Product Specifications and Parameters

Biological Background: MZB1 Function and Localization

• MZB1, also known as pERp1, MEDA-7, and PACAP, is a marginal zone B- and B1-cell-specific protein encoded by the MZB1 gene. Isoform 2 is generated by alternative splicing and may regulate apoptosis.
• Acts as a molecular chaperone or oxidoreductase, associating with IgM heavy and light chains to promote IgM assembly and secretion; it displays low oxidoreductase activity (By similarity).
• Functions as a hormone-regulated adipokine/pro-inflammatory cytokine implicated in chronic inflammation, cellular expansion, and blunted insulin response in adipocytes, potentially contributing to insulin resistance.
• Diversifies peripheral B-cell functions by regulating Ca²⁺ stores, antibody secretion, and integrin activation.
• Localizes predominantly to the endoplasmic reticulum lumen, but is also found secreted and in the cytoplasm.
• Widely expressed, with highest levels in adult brain, small intestine, thymus, and spleen; expression is frequently lower in intestinal-type gastric cancer, and in obese patients, more abundant in omental than subcutaneous fat.
• Key structural features include a signal peptide, disulfide bonds, and a determined three-dimensional structure.

Experimental Guidance and Technical Tips

• For Western blot, target detection at ~21 kDa under reducing conditions is typical. Consider using a positive control such as lysates from lymphoid tissues or MZB1-expressing cell lines.
• For immunohistochemistry (IHC-P), antigen retrieval with citrate or EDTA buffer may enhance signal due to ER localization. Validate in appropriate tissue sections (e.g., spleen, thymus).
• For ELISA, the antibody can be used as either capture or detection reagent in sandwich assays, paired with a matched monoclonal antibody if available.
• Cross-reactivity with mouse and rat is confirmed; verify performance in your specific sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research labs to streamline IVD raw-material procurement, offering validated antibody pairs, optimized polyclonal antibodies like this anti-MZB1, and bulk ancillary reagents. For projects targeting ER chaperones or B-cell markers, our team can source rare raw materials to bridge your concept-to-clinic pipeline.