Anti-MYSM1 Rabbit Polyclonal Antibody for WB, ELISA - Q5VVJ2

Core Product Specifications and Parameters

Biological Background: MYSM1 Function and Localization

• MYSM1 is a deubiquitinase metalloprotease that plays essential regulatory roles in hematopoietic stem cell function, blood cell production, and immune response. Related references: PMID:24062447 PMID:26220525 PMID:28115216
• In the cytoplasm, MYSM1 represses innate immunity and autoimmunity by suppressing the cGAS-STING1 signaling pathway through 'Lys-63'-linked deubiquitination of STING1. Related references: PMID:33086059
• Within the nucleus, MYSM1 acts as a transcriptional regulator by deubiquitinating monoubiquitinated histone H2A (uH2A), leading to dissociation of histone H1 and derepression of genes such as EBF1, PAX5, ID2, and FLT3. Related references: PMID:17707232 PMID:24062447
• MYSM1 attenuates NOD2-mediated inflammation by deubiquitinating RIPK2, a process involving 'Lys-63'-linked polyubiquitin chains.
• The protein localizes to both the nucleus and the cytoplasm, reflecting its dual functions in transcriptional regulation and cytoplasmic signaling.
• Structural features include a SWIRM domain, an MPN domain with metalloprotease activity, and a zinc-binding motif; it is classified as a metalloprotease and is linked to chromatin regulation, transcription, and the ubiquitin conjugation pathway.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 720–821 of human MYSM1, near the C-terminus. Conservation of this region in mouse and rat orthologs should be verified to support expected cross-reactivity.
• For Western blot (WB), the predicted molecular weight is 95 kDa; validate band detection in relevant lysates (e.g., hematopoietic cell lines, immune tissues) and optimize antibody dilution empirically.
• For ELISA, consider using the antibody as a capture or detection reagent; validation with recombinant MYSM1 or cell lysates in appropriate buffer systems is recommended.
• Documented cross-reactivity includes human, mouse, and rat; for other species, perform sequence alignment of the immunogen region before use.
• As a polyclonal antibody, lot-to-lot consistency should be verified for critical quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibodies, bulk reagents, and customized procurement solutions. This anti-MYSM1 antibody supports research in deubiquitinase pathways, hematopoietic biology, and immune regulation. For your complete project needs—from rare target sourcing to ancillary reagent supply—partner with CamelBio to streamline your development pipeline.