Anti-MYRF Polyclonal Antibody for WB and ELISA - Q9Y2G1

Core Product Specifications and Parameters

Biological Background: MYRF Function and Localization

• MYRF is a precursor transcription factor that undergoes autocatalytic cleavage to release an N-terminal component, which acts as a transcription factor specifically activating CNS myelin genes. Related references: PMID:23966832
• The membrane-bound C-terminal part remains anchored to the endoplasmic reticulum membrane and has no transcription factor activity.
• It is a key regulator of oligodendrocyte maturation and CNS myelination, directly activating expression of myelin genes such as MBP, MOG, MAG, DUSP15, and PLP1. Related references: PMID:23966832
• MYRF is required not only during oligodendrocyte differentiation but also for ongoing maintenance of myelin gene expression and the mature oligodendrocyte phenotype. Related references: PMID:23966832
• The protein localizes to the endoplasmic reticulum membrane, nucleus, and cytoplasm.
• Tissue expression is highest in lung, brainstem, uterus, and retinal pigment epithelium, with lower levels in basal ganglion, liver, cerebellum, choroid, and optic nerve. Related references: PMID:10828591, PMID:31048900
• Features include an autocatalytic protease domain, DNA-binding domain, transmembrane helix, and coiled-coil region; it is subject to acetylation and glycosylation.
• Mutations in MYRF are associated with disease variants, highlighting its critical role in CNS myelination and development.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight is 124 kDa; consider using appropriate gel percentage and transfer conditions for high molecular weight proteins.
• The immunogen is the N-terminal region; the antibody may detect both the full-length precursor and the cleaved N-terminal transcription factor.
• Validate reactivity in human and mouse CNS tissue lysates or oligodendrocyte cell lines, where MYRF expression is enriched.
• For ELISA, titrate the antibody against the immunogen peptide or recombinant MYRF fragments to establish optimal working concentration.
• Be aware that MYRF undergoes autocatalytic cleavage; pretreatment of samples may influence detection of different forms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio acts as a comprehensive sourcing partner for IVD raw materials, providing reliable access to validated antibody pairs, optimized polyclonal antibodies like this anti-MYRF reagent, bulk ancillary reagents, and even rare-target raw materials to support CNS myelination and transcription research. Our global supply network and technical expertise enable diagnostic manufacturers and laboratories to efficiently procure high-quality materials from discovery through clinical application.