Anti-MYPN Rabbit Polyclonal Antibody for WB, ELISA - Q86TC9

Core Product Specifications and Parameters

Biological Background: Myopalladin Function and Localization

• Myopalladin (MYPN) is a 145 kDa sarcomeric protein with immunoglobulin (Ig) domain repeats and a coiled-coil region, encoded by the MYPN gene.
• It acts as a structural linker, tethering nebulin (in skeletal muscle) or nebulette (in cardiac muscle) to alpha-actinin at the Z-lines, maintaining sarcomere integrity.
• MYPN localizes to the cytoplasm, nucleus, and specifically the Z-line of the sarcomere in striated muscle cells.
• Tissue expression is predominantly in adult skeletal muscle and fetal heart, consistent with its role in muscle architecture.
• The protein undergoes alternative splicing, generating multiple isoforms, and contains phosphorylation and disulfide bond sites.
• Mutations in MYPN are associated with human cardiomyopathies (CMH22, CMD1DD) and nemaline myopathy (NEM11), highlighting its importance in muscle disease.
• Functional domains include Ig-like repeats and a coiled-coil region, enabling protein-protein interactions with actin-binding partners.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-250 of human MYPN, a region near the N-terminus. When using this antibody, consider epitope accessibility in native folded proteins and validate in your specific sample system.
• For Western blot (WB), the predicted molecular weight is ~145 kDa. Validate the antibody in human and mouse muscle tissue lysates, and optimize blocking and detection conditions.
• For ELISA, consider using recombinant MYPN protein as a positive control. Titration of the antibody and optimization of coating buffers are recommended to achieve optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to high-quality IVD raw materials, including validated antibodies for sarcomere and muscle disease targets. Beyond this anti-MYPN polyclonal antibody, we offer antibody pairs, monoclonal options, and ancillary reagents to accelerate your diagnostic or research workflow. Our supply chain supports bulk orders and rare-target sourcing, ensuring seamless integration from concept to clinic.