Anti-Myotubularin (MTM1) Polyclonal Antibody for WB, ELISA - Q13496

Core Product Specifications and Parameters

Biological Background: Myotubularin Function and Localization

• Myotubularin (MTM1) is a dual-specificity phosphatase that primarily acts as a lipid phosphatase, dephosphorylating phosphatidylinositol 3-monophosphate (PI3P) and phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2). Related references: PMID:10900271, PMID:11001925, PMID:12646134
• It also exhibits protein phosphatase activity toward phosphotyrosine- and phosphoserine-containing peptides. Related references: PMID:9537414
• MTM1 negatively regulates epidermal growth factor receptor (EGFR) degradation by controlling EGFR trafficking from late endosomes to lysosomes. Related references: PMID:14722070
• The protein is involved in vacuolar formation and morphology, and plays roles in desmin intermediate filament assembly, mitochondrial morphology, and positioning. Related references: PMID:21135508
• In skeletal muscle, MTM1 is required for maintenance but not for myogenesis, and it stabilizes the protein levels of MTMR12. Related references: PMID:21135508, PMID:23818870
• Subcellularly, MTM1 localizes to the cytoplasm, cell membrane, cell projections (filopodia and ruffles), late endosomes, and specifically to myofibrils and sarcomeres in muscle cells.
• Mutations in the MTM1 gene are linked to X-linked myotubular myopathy (XLMTM), a severe congenital muscle disorder.
• The MTM1 gene undergoes alternative splicing, producing multiple isoforms, and is subject to phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen used for this rabbit polyclonal antibody corresponds to the C-terminal region (amino acids 484-603) of human myotubularin. This design may enable detection of full-length protein and certain C-terminal-containing splice variants.
• For Western blot (WB), a band around 70 kDa is expected for the full-length myotubularin in human, mouse, or rat samples. Consider using a positive control like HeLa or muscle tissue lysates and validate in your specific sample system.
• In ELISA applications, this antibody could be employed as a capture or detection reagent; specificity verification against the immunogen or recombinant protein is advisable.
• Pre-adsorption with the immunogen peptide or recombinant protein may help confirm band specificity in WB.
• Since the antibody cross-reacts with mouse and rat myotubularin, it may be useful for translational studies in these model organisms; however, validation in the relevant sample context is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, offering validated antibody pairs, bulk ancillary reagents, and rare target procurement to streamline your assay development. This anti-myotubularin polyclonal antibody supports research in phosphoinositide metabolism, endosomal trafficking, and muscle disease, complementing our extensive portfolio for neuromuscular disorder studies. With CamelBio, you gain access to a comprehensive supply chain and expert technical support from concept to clinic.