Anti-Myoglobin Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P02144

Core Product Specifications and Parameters

Biological Background: Myoglobin Function and Localization

• Monomeric heme protein primarily responsible for oxygen storage and facilitated diffusion in muscle tissues. Reversibly binds oxygen via a pentacoordinated heme iron, enabling timely release during periods of heightened demand. Related references: PMID:30918256 PMID:34679218
• Exhibits nitrite reductase activity, regulating bioactive nitric oxide production depending on tissue oxidative conditions. Related references: PMID:32891753
• Protects cells against reactive oxygen species under stress conditions such as hypoxia and anoxia through its pseudoperoxidase activity. Related references: PMID:34679218
• Localized to the cytoplasm and sarcoplasm of muscle cells.
• Also known as nitrite reductase MB and pseudoperoxidase MB, reflecting its enzymatic side activities.
• Classified under muscle protein, heme, iron-binding, and is associated with disease variants and phosphorylation.

Experimental Guidance and Technical Tips

• For Western blot, consider using reduced sample preparation as the immunogen was a recombinant full-length protein; validate in relevant muscle lysates (e.g., skeletal or cardiac muscle).
• In immunofluorescence (IF-P), optimize fixation and permeabilization conditions given the cytoplasmic localization of myoglobin.
• For ELISA, the antibody may be used for detection; pairing with a capture antibody could be explored for sandwich assay development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and laboratories with a one-stop supply of IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies like this anti-Myoglobin product. We support muscle biology, cardiovascular marker, and oxygen transport research with bulk ancillary reagents and rare target raw-material sourcing—bridging your needs from concept to clinic. Contact us for customized supply solutions.