Anti-Myoglobin Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P02144

Core Product Specifications and Parameters

Biological Background: Myoglobin Function and Localization

• Myoglobin is a monomeric heme protein that functions as the primary oxygen storage and transport molecule in muscle tissues, facilitating oxygen diffusion and release during increased metabolic demand (PubMed:30918256, PubMed:34679218). Related references: PMID:30918256 PMID:34679218
• Under oxidative stress conditions, myoglobin exhibits pseudoperoxidase activity, protecting cells against reactive oxygen species (PubMed:34679218). Related references: PMID:34679218
• It also possesses nitrite reductase activity, regulating bioactive nitric oxide production in a redox-dependent manner (PubMed:32891753). Related references: PMID:32891753
• The protein localizes to the cytoplasm and sarcoplasm of muscle cells, consistent with its role in oxygen management within muscle fibers.
• As a phosphoprotein with a heme iron binding site, myoglobin is subject to post-translational modifications that may regulate its function.
• Classified as a muscle protein and member of the globin family, myoglobin is essential for normal muscle physiology.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a recombinant fusion protein spanning amino acids 2–154 of human myoglobin, covering nearly the full-length protein. This design may confer recognition of native myoglobin in various assays, but validation in the relevant sample system is recommended.
• For Western blot (WB) analysis, a band around 17 kDa is expected; consider using muscle tissue lysates (e.g., skeletal or cardiac muscle) or myoglobin-expressing cell lines as positive controls.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF-P), test the antibody on fixed muscle tissue sections. Antigen retrieval conditions may need optimization, and appropriate controls (e.g., non-muscle tissue) should be included.
• Due to the cross-reactivity with mouse and rat myoglobin, this antibody can be employed in translational studies involving these species, but the reactivity profile should be verified in your specific experimental model.
• The antibody is also suitable for ELISA applications; pairing with a capture antibody or using as a detection reagent could be explored for quantitative myoglobin measurement.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutions with one-stop access to IVD raw materials, streamlining the journey from concept to clinic. This anti-myoglobin monoclonal antibody supports applications in muscle biology, oxygen transport research, and cardiac or skeletal muscle injury biomarker studies. Beyond individual reagents, CamelBio offers validated antibody pairs, bulk monoclonal and polyclonal antibodies, ancillary reagents, and customized sourcing for rare targets, ensuring reliable material supply for assay development and production scale-up.