Anti-MYOF Rabbit Polyclonal Antibody (KO Validated) for WB, ELISA - Q9NZM1

Core Product Specifications and Parameters

Biological Background: Myoferlin Function and Localization

• Myoferlin (also known as Fer-1-like protein 3) is a calcium/phospholipid-binding protein encoded by the MYOF gene, and is a member of the ferlin family of proteins involved in membrane fusion and repair.
• It plays a critical role in the plasmalemma repair mechanism of endothelial cells, enabling rapid resealing of membranes disrupted by mechanical stress.
• Involved in endocytic recycling and implicated in VEGF signal transduction by regulating the levels of the receptor KDR.
• Localizes to the cell membrane, nucleus membrane, and cytoplasmic vesicle membranes, including late endosome membranes.
• Expressed in myoblast and endothelial cells, with high expression in cardiac and skeletal muscles; also present in lung, and at very low levels in kidney, placenta, and brain.
• Contains multiple C2 domains that mediate calcium-dependent phospholipid binding, and is subject to post-translational modifications including acetylation and phosphorylation.

Experimental Guidance and Technical Tips

The immunogen used for this antibody is a recombinant fusion protein corresponding to the N-terminal region (aa 1-190) of human MYOF. This design may allow recognition of multiple isoforms or splice variants; we recommend verifying specificity in the relevant sample system. For Western blot, note the high molecular weight of myoferlin (~235 kDa); consider using optimized transfer conditions and a suitable membrane for efficient detection. In ELISA assays, titer and working dilution should be empirically determined. As a polyclonal antibody, lot-to-lot consistency may vary; validation of each new lot in your specific application is advisable.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your myoferlin research and assay development needs. With a focus on membrane repair and muscle biology, CamelBio is your partner for reliable, high-quality reagents.