Anti-MYO5B Rabbit Polyclonal Antibody for WB, IF-P, ELISA - Q9ULV0

Core Product Specifications and Parameters

Biological Background: MYO5B Function and Localization

• MYO5B (unconventional myosin-Vb) is an actin-based motor protein involved in intracellular vesicular trafficking along actin filaments.
• It associates with the CART complex and is necessary for efficient transferrin receptor recycling, preventing EGFR degradation.
• MYO5B, together with RAB11A and RAB11FIP2, mediates the transport of NPC1L1 to the plasma membrane, and also participates in CFTR trafficking and epithelial cell polarization.
• Required for proper localization of the bile salt export pump ABCB11 at the apical/canalicular plasma membrane of hepatocytes. Related references: PMID:34816459
• Localizes predominantly in the cytoplasm and is involved in transcytosis regulation with RAB25.
• Alternative splicing generates multiple isoforms; post-translational modifications include acetylation and phosphorylation.
• Mutations in MYO5B are associated with microvillus inclusion disease (MVID) and progressive familial intrahepatic cholestasis type 10 (PFIC10), highlighting its role in epithelial integrity and bile acid homeostasis.

Experimental Guidance and Technical Tips

• The immunogen (C-terminal region, aa 1440-1690) may enable detection of specific MYO5B isoforms or splice variants; consider verifying isoform recognition via sequence alignment.
• For Western blotting, expect a predominant band near 214 kDa; optimize transfer conditions for high-molecular-weight proteins and use appropriate molecular weight markers.
• In immunofluorescence (IF-P), MYO5B typically shows cytoplasmic vesicular distribution; permeabilization with Triton X-100 or saponin is recommended for intracellular staining.
• ELISA can be used for quantitative detection; validate linearity and sensitivity in the relevant sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material sourcing with a comprehensive portfolio of validated antibodies, antibody pairs, and bulk ancillary reagents. For researchers investigating MYO5B in vesicular trafficking, epithelial polarity, or cholestatic diseases, CamelBio offers tailored supply solutions from lead identification to commercial scale. Inquire about custom monoclonal or recombinant antibody development for challenging targets.