Anti-MYO1D Polyclonal Antibody for WB and ELISA - O94832

Core Product Specifications and Parameters

Biological Background: MYO1D Function and Localization

• Unconventional myosin-Id is an actin-based motor protein with ATPase activity, belonging to the myosin superfamily of molecular motors.
• It plays a critical role in endosomal protein trafficking, particularly in the transfer of cargo proteins from early to recycling endosomes.
• MYO1D is required for normal planar cell polarity in ciliated tracheal cells and for proper rotational polarity and coordinated unidirectional ciliary movement.
• The protein localizes to the cytoplasm, perikaryon, dendrites, early endosomes, and the cell cortex, reflecting its diverse cellular roles.
• It is widely expressed across tissues, with highest levels in brain, lung, and ovary, and lowest in spleen.
• Key structural features include an N-terminal motor domain with actin-binding and ATP-binding sites, a neck region with calmodulin-binding IQ motifs, and a tail domain involved in cargo interaction.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its activity and localization.
• This antibody targets amino acids 180-380 of human MYO1D, corresponding to part of the motor domain, making it suitable for detecting the protein in various species.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein from the internal region (aa 180-380) of human MYO1D, chosen to potentially recognize multiple isoforms and processed forms.
• For western blot (WB), anticipate a band at approximately 116 kDa; validate specificity using relevant positive controls like brain, lung, or ovary lysates.
• This antibody is reported to cross-react with mouse, making it suitable for studies in mouse disease models; always confirm reactivity in your specific experimental context.
• For ELISA, consider testing serial dilutions of the antibody against the recombinant immunogen or endogenous protein from tissue extracts.
• Given the protein's role in endosomal trafficking and ciliary polarity, appropriate cellular models include epithelial cells, neuronal cells, or ciliated tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, technical services, and consulting for diagnostic manufacturers, labs, and research institutes. This anti-MYO1D polyclonal antibody is ideal for exploring endosomal trafficking and ciliary biology in both human and mouse systems. Our supply capabilities include validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assistance in sourcing rare target raw materials to accelerate your project from concept to clinic.