Anti-MYO1C Rabbit Monoclonal Antibody for WB, IF/ICC, IHC-P - O00159

Core Product Specifications and Parameters

Biological Background: MYO1C Function and Localization

• MYO1C (myosin-Ic) is an unconventional myosin motor protein that uses actin-based ATPase activity to generate force and movement, linking cellular membranes to the actin cytoskeleton.
• It plays a critical role in insulin-stimulated glucose transporter recycling by regulating the translocation of GLUT4-containing vesicles to the plasma membrane.
• As a component of the hair cell adaptation-motor complex, MYO1C mediates adaptation of mechanoelectrical transduction in stereocilia of vestibular hair cells.
• MYO1C participates in transcriptional regulation by associating with active ribosomal genes and cooperating with the WICH chromatin-remodeling complex to facilitate transcription initiation.
• The protein localizes to multiple subcellular sites, including the cytoplasm, nucleus, cell cortex, stereocilia membrane, cytoplasmic vesicles, and ruffle membrane.
• Post-translational modifications include acetylation, methylation, and phosphorylation; it contains conserved domains for ATP binding, actin binding, and calmodulin binding.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal tail domain (aa 769-1028) of human MYO1C, a region divergent among myosin classes, which likely contributes to target specificity.
• For Western blot, the predicted molecular weight is 122 kDa; use appropriate gel percentage and verify the band size with molecular weight markers.
• For immunofluorescence and immunohistochemistry, permeabilization is recommended to detect both nuclear and cytoplasmic localizations. Validate in the relevant sample system and include appropriate controls.
• The antibody cross-reacts with mouse and rat, but species-specific validation may be required for certain applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides this MYO1C monoclonal antibody as part of our comprehensive IVD raw-material portfolio, supporting research into cytoskeletal dynamics, insulin signaling, and hearing adaptation. We offer diagnostic manufacturers and labs validated antibody pairs, bulk ancillary reagents, and tailored sourcing for rare-target raw materials. Contact us to streamline your procurement from concept to clinic.