Anti-MYO1C Polyclonal Antibody for WB, IHC-P, IP, ELISA - O00159

Core Product Specifications and Parameters

Biological Background: MYO1C Function and Localization

• MYO1C encodes unconventional myosin-Ic, also known as myosin I beta (MMI-beta, MMIb), an actin-based motor molecule with ATPase activity.
• Its tail domain mediates binding to phosphoinositides and links the actin cytoskeleton to cellular membranes, enabling intracellular movements such as glucose transporter (GLUT4) vesicle recycling in response to insulin.
• In hair cells of the inner ear, MYO1C is a component of the adaptation-motor complex, mediating mechanoelectrical transduction adaptation in stereocilia.
• MYO1C also localizes to the nucleus, where it associates with transcriptionally active ribosomal genes and facilitates transcription initiation by cooperating with the WICH chromatin-remodeling complex.
• Subcellularly, MYO1C is found in the cytoplasm, nucleus (nucleoplasm, nucleolus), cell cortex, stereocilium membrane, cytoplasmic vesicles, and ruffle membrane.
• The protein is subject to post-translational modifications including acetylation, methylation, and phosphorylation, and contains actin-binding, ATP-binding, and calmodulin-binding domains.

Experimental Guidance and Technical Tips

• The immunogen used spans amino acids 769-1028 of human MYO1C, corresponding to the C-terminal tail domain, which may confer target specificity.
• For Western blot, the predicted molecular weight is 122 kDa; consider including appropriate molecular weight markers and optimizing blocking and antibody incubation conditions.
• In immunohistochemistry (paraffin), antigen retrieval and antibody titration may be necessary for optimal signal in human, mouse, or rat tissues.
• For immunoprecipitation and ELISA, validate the antibody's performance in the relevant sample system and consider using positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop sourcing for IVD raw materials. Our portfolio includes validated polyclonal antibodies like this MYO1C rabbit pAb, ideal for studying cytoskeletal dynamics and transcriptional regulation. From optimized antibody pairs to bulk ancillary reagents, we support your entire development pipeline—from concept to clinic.