Anti-MYO1B Rabbit Monoclonal Antibody for WB, ELISA - O43795

Core Product Specifications and Parameters

Biological Background: MYO1B Function and Localization

• MYO1B (Unconventional myosin-Ib) is a motor protein also designated as MYH-1c, MMI-alpha, or MMIa.
• Encoded by the MYO1B gene, it belongs to the myosin superfamily of actin-based motor molecules.
• FUNCTION: Participates in processes critical to neuronal development and function, including cell migration, neurite outgrowth, and vesicular transport.
• KEYWORDS: Actin-binding, ATP-binding, calmodulin-binding, alternative splicing, phosphoprotein, and Ubl conjugation.
• Structural domains: Contains a conserved motor head domain, a neck region with calmodulin-binding IQ motifs, and a tail domain likely mediating cargo interactions.
• Localization: While not explicitly annotated, unconventional myosins typically associate with the actin cytoskeleton and intracellular membranes.
• Post-translational modifications: Predicted phosphoprotein; undergoes isopeptide bond formation and ubiquitination.
• Tissue specificity: Data not provided, but functional implications point to the nervous system.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment spanning residues 916–1136 of human MYO1B, located in the C-terminal tail domain; consider this when interpreting epitope accessibility.
• For Western blotting, the predicted molecular weight is ~132 kDa. Validate with positive controls from human, mouse, or rat neuronal cell lines or tissue lysates.
• For ELISA applications, titrate the antibody as a capture or detection reagent according to your assay setup; cross-reactivity with mouse and rat MYO1B supports multi-species studies.
• Due to the absence of reported subcellular fractionation data, consider confirming localization via complementary methods such as immunofluorescence.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic developers and research teams to streamline the procurement of high-quality IVD raw materials. For neuroscience and motor protein research, we offer validated monoclonal antibodies like anti-MYO1B, along with bulk ancillary reagents and technical consultation. Whether you require established antibody pairs, custom antibody generation, or hard-to-find targets, our sourcing platform helps bridge gaps from concept to clinical application.