Anti-MYO19 Rabbit Monoclonal Antibody for WB, ELISA - Q96H55

Core Product Specifications and Parameters

Biological Background: MYO19 Function and Localization

• MYO19, also known as unconventional myosin-XIX or myosin head domain-containing protein 1 (MYOHD1), is an actin-based motor protein belonging to the myosin superfamily.
• It possesses ATPase activity and moves toward the plus-end of actin filaments, localizing to the mitochondrial outer membrane (PubMed:19932026, PubMed:23568824, PubMed:25447992). Related references: PMID:19932026 PMID:23568824 PMID:25447992
• MYO19 is essential for mitochondrial inheritance during mitosis, ensuring proper distribution of mitochondria to daughter cells (PubMed:25447992). Related references: PMID:25447992
• Subcellularly, it is anchored at the mitochondrial outer membrane, and also detected in the cytoplasm and associated with the cytoskeleton.
• The protein is expressed widely across various tissues and cell lines.
• Post-translational modifications include phosphorylation, and it contains hallmark myosin repeat domains; it is classified under keywords such as actin-binding, ATP-binding, and mitochondrial membrane protein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 450–650) of human MYO19; this recombinant fragment likely presents conformational epitopes suitable for both WB and ELISA applications.
• For Western blot, consider using whole cell or mitochondrial-enriched lysates. The expected molecular weight is approximately 109 kDa. Verify specificity by knockdown or knockout controls where possible.
• For ELISA, titrate the antibody against the recombinant protein or cell lysates. Pair with a suitable detection antibody for sandwich ELISA format if developing an assay.
• Cross-reactivity is reported for human, mouse, rat, and monkey; validate performance in the relevant species and sample type prior to routine use.
• As a monoclonal antibody, lot-to-lot consistency is expected, but always run internal validation for critical diagnostic applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement by offering validated MYO19 antibodies and a broad portfolio of monoclonal/polyclonal antibodies for mitochondrial research targets. Our supply network includes optimized antibody pairs, bulk ancillary reagents, and rare target sourcing services to support diagnostic manufacturers from concept to clinic. Partner with CamelBio to secure the critical raw materials you need for robust assay development.