Anti-MYO18A Rabbit Polyclonal Antibody for WB and ELISA - Q92614

Core Product Specifications and Parameters

Biological Background: MYO18A Function and Localization

• MYO18A encodes unconventional myosin-XVIIIa, a large multidomain motor protein also known as MAJN, SPR210, or MYSPDZ.
• Plays a key role in linking Golgi membranes to the cytoskeleton, participating in the tensile force required for vesicle budding and thereby contributing to Golgi morphology and trafficking. Related references: PMID:19837035, PMID:23345592
• In cooperation with LURAP1 and CDC42BPA/CDC42BPB, modulates lamellar actomyosin retrograde flow, which is crucial for cell protrusion and migration. Related references: PMID:18854160
• Regulates trafficking, expression, and activation of innate immune receptors on macrophages; suppresses inflammatory responsiveness by modulating CD14 trafficking. Related references: PMID:25965346
• Acts as a receptor for surfactant protein A (SFTPA1/SP-A), mediating internalization and clearance of SFTPA1-opsonized S. aureus by alveolar macrophages. Related references: PMID:16087679, PMID:21123169
• Strongly enhances natural killer cell cytotoxicity, highlighting its role in innate immune effector functions. Related references: PMID:27467939
• Subcellular localization includes the Golgi apparatus (especially trans-Golgi network), endoplasmic reticulum-Golgi intermediate compartment, cytoskeleton, and cell surface.
• The protein contains characteristic myosin domains (motor domain, coiled-coil) and undergoes alternative splicing; it is classified as an ATP-binding, nucleotide-binding motor protein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 1970–2054), a region that may be conserved across known isoforms; consider verifying isoform detection in your system.
• For Western blot, a low-percentage (e.g., 6%) SDS-PAGE gel is recommended to resolve the full-length 233 kDa protein; extended transfer times may be needed.
• Validate antibody specificity in the relevant sample system using positive and negative controls; cross-reactivity is reported for human, mouse, and rat.
• ELISA conditions should be optimized individually; check for potential nonspecific binding common to polyclonal preparations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized IVD raw-material partner offering one-stop access to antibodies, antigens, enzymes, and ancillary reagents. This anti-MYO18A polyclonal antibody supports research in cytoskeletal dynamics, Golgi biology, and innate immunity. Beyond the listed product, we can source validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and even rare-target raw materials to streamline your diagnostic or research workflows.