Anti-MYO10 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9HD67

Core Product Specifications and Parameters

Biological Background: MYO10 Function and Localization

• MYO10, also known as unconventional myosin-X or myosin-10, is an actin-based motor protein with ATPase activity. It binds to actin filaments and bundles, functioning as a plus end-directed motor with enhanced velocity on actin bundles (PubMed:27580874).
  Related references:
  PMID:27580874
• Its tail domain binds to membranous compartments containing phosphatidylinositol 3,4,5-trisphosphate (PIP3) or integrins, mediating cargo transport along actin filaments. This interaction regulates cell shape, spreading, and adhesion.
• MYO10 stimulates filopodia formation and elongation. In hippocampal neurons, it induces dendritic filopodia by trafficking VASP to filopodial tips, promoting actin elongation.
• An alternative isoform (isoform 2) functions as a dominant-negative regulator, suppressing filopodia-inducing and axon outgrowth-promoting activities of isoform 1, and instead promoting spine formation and spine head expansion in hippocampal neurons.
• Subcellular localization includes cytoplasm, cytosol, cell projections such as lamellipodia, ruffles, and filopodial tips, as well as the cell cortex and filopodial membrane.
• The protein undergoes post-translational modifications including acetylation and phosphorylation. It contains multiple domains: a coiled-coil region, calmodulin-binding motifs, and a pleckstrin homology (PH) domain.
• MYO10 is ubiquitously expressed across tissues, consistent with its fundamental role in cell morphology and migration.
• Keywords associated with MYO10 include: 3D-structure, Actin-binding, Alternative splicing, ATP-binding, Calmodulin-binding, Motor protein, Myosin, Nucleotide-binding, Phosphoprotein, Transport.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a sequence in the tail domain of MYO10 (residues 845–944), which is present in both isoform 1 and likely isoform 2. The antibody may recognize both isoforms, but validation in the relevant sample system is recommended.
• For Western blotting, a band of approximately 237 kDa is expected. Use an appropriate protein ladder for high-molecular-weight proteins and optimize transfer conditions.
• In immunofluorescence/immunocytochemistry, MYO10 localizes to filopodia tips, lamellipodia, and ruffles. Validate fixation and permeabilization protocols to preserve delicate cell projections.
• For ELISA, ensure that the coating antigen is compatible with the immunogen region. Cross-reactivity with mouse is reported, supporting its use in mouse-derived samples.

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