Anti-MYLIP Polyclonal Antibody for WB, ELISA - Q8WY64

Core Product Specifications and Parameters

Biological Background: MYLIP Function and Localization

• MYLIP (also known as IDOL, MIR) is an E3 ubiquitin-protein ligase with a RING-type zinc finger domain, mediating ubiquitination of target proteins.
• It plays a critical role in cholesterol homeostasis by ubiquitinating and targeting the LDL receptor (LDLR), VLDLR, and LRP8 for proteasomal degradation in a sterol-dependent manner.
• Additionally, MYLIP ubiquitinates myosin regulatory light chain (MRLC), inhibiting neurite outgrowth in the presence of NGF.
• The protein contains a FERM domain, a RING finger domain, and a C-terminal IDOL domain, which coordinate substrate recognition and E2 enzyme interaction (mainly UBE2D family).
• Alternative splicing produces multiple isoforms, contributing to functional diversity.
• MYLIP is localized in the cytoplasm and at the cell membrane.
• It is ubiquitously expressed across tissues.
• Keywords: Transferase, Ubl conjugation pathway, metal-binding, zinc-finger, alternative splicing.

Experimental Guidance and Technical Tips

• For Western blotting, a band around 50 kDa is expected under reducing conditions. Validate expression in relevant cell or tissue lysates using appropriate positive and negative controls.
• In ELISA applications, consider direct or sandwich formats depending on assay design; users should optimize coating conditions and antibody titrations.
• As a polyclonal antibody, batch-to-batch consistency should be verified, and it is recommended to include a standard curve or reference sample in each experiment.
• The immunogen corresponds to amino acids 266-445, spanning a region within the FERM and IDOL domains; this may be useful for assays requiring domain-specific recognition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents for cholesterol metabolism research. We also specialize in sourcing rare-target raw materials like MYLIP E3 ligase antibodies to support your assay development from concept to clinic.