Anti-MYL9 Rabbit pAb for WB, IF-P, ELISA - P24844

Core Product Specifications and Parameters

Biological Background: MYL9 Function and Localization

• Myosin regulatory light polypeptide 9 (MYL9), also known as 20 kDa myosin light chain (LC20), MLC-2C, myosin regulatory light chain MRLC1, is a regulatory subunit of myosin.
• Plays a critical regulatory role in smooth muscle and nonmuscle cell contractile activity via phosphorylation; involved in cytokinesis, receptor capping, and cell locomotion. Related references: PMID:11942626 PMID:2526655
• In myoblasts, may regulate PIEZO1-dependent cortical actomyosin assembly important for myotube formation (by similarity).
• Subcellular localization: Found in the cytoplasm, associated with the cytoskeleton and cell cortex.
• Tissue specificity: Expressed in smooth muscle tissues and in a subset of nonmuscle cells.
• Keywords highlight post-translational modifications including acetylation and phosphorylation, calcium and metal binding, and classification as a motor protein and muscle protein.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1-100 of human MYL9 (NP_006088.2); antibodies may recognize epitopes within the N-terminal region. For western blot, a band around 20 kDa is expected; optimize lysis and loading controls for your sample type.
• As a polyclonal antibody, batch-to-batch variation is possible; validate performance in your specific assay system with appropriate positive and negative controls.
• For immunofluorescence (IF-P), optimize fixation and permeabilization for cortical or cytoskeletal detection; co-staining with actin or myosin markers can confirm localization.
• The antibody is reported for WB, IF-P, and ELISA and cross-reacts with human, mouse, and rat samples; confirm cross-reactivity experimentally if used in other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive sourcing platform for IVD raw materials, including this anti-MYL9 polyclonal antibody. Beyond individual research-grade antibodies, we provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing support. For scientists developing assays in smooth muscle biology or contractility research, CamelBio streamlines the transition from concept to clinic with reliable supply and technical resources.