Anti-MYL6B Rabbit Polyclonal Antibody (KO Validated) for WB and ELISA - P14649

Core Product Specifications and Parameters

Biological Background: MYL6B Function and Localization

• Myosin light chain 6B (MYL6B) functions as a regulatory light chain of myosin, a motor protein essential for force generation.
• It does not bind calcium, differentiating it from other myosin light chains involved in calcium-dependent regulation.
• Alternative names include Myosin light chain 1 slow-twitch muscle A isoform (MLC1sa) and Smooth muscle and nonmuscle myosin light chain alkali 6B, indicating tissue-specific isoforms.
• MYL6B is classified under keywords such as Motor protein, Muscle protein, and Myosin, reflecting its role in contractile systems.
• The calculated molecular weight is 22764 Da, consistent with small myosin subunits.
• The protein contains repeat regions (Repeat keyword), suggesting structural motifs important for interaction or stability.
• A 3D structure has been determined (3D-structure keyword), providing insights into its molecular conformation.
• It has been identified in proteomics studies (Proteomics identification keyword), confirming its expression in human cells.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human MYL6B (aa 1-208), which should elicit antibodies recognizing both recombinant and endogenous protein.
• For Western blotting, expect a band near 23 kDa; validate performance using positive control lysates from human, mouse, or rat tissues (e.g., skeletal muscle or smooth muscle).
• For ELISA, consider coating with recombinant MYL6B antigen and testing serial dilutions of antibody; optimize blocking and detection conditions for your specific assay format.
• Since the antibody is KO validated, include knockout cell lysates as a negative control to confirm specificity in your system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop partner for IVD raw materials, offering this anti-MYL6B polyclonal antibody alongside a wide range of validated antibodies and reagents. Whether you need antibody pairs, recombinant proteins, or bulk ancillary supplies for muscle biology research or assay development, our sourcing team supports your journey from concept to clinic.