Anti-MYL3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P08590

Core Product Specifications and Parameters

Biological Background: MYL3 Function and Localization

• MYL3 encodes myosin light chain 3, a regulatory light chain of myosin that does not bind calcium.
• It is also known as cardiac myosin light chain 1 (CMLC1) and ventricular/slow twitch myosin alkali light chain, indicating predominant expression in cardiac and slow-twitch skeletal muscle.
• MYL3 is a component of the myosin hexamer, contributing to the structural integrity and regulation of the sarcomere.
• The protein undergoes post-translational modifications, including methylation and phosphorylation, which may modulate its function and interactions.
• Mutations in MYL3 are linked to cardiomyopathy, highlighting its essential role in cardiac muscle physiology.
• The predicted molecular weight of the mature protein is approximately 22 kDa (21932 Da).

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1-195 of human MYL3, covering nearly the entire protein, which can enhance antibody reactivity against native conformations.
• For Western blot (WB), consider loading cardiac or slow-twitch muscle lysates to enrich the target; expect a band near 22 kDa.
• In immunofluorescence/immunocytochemistry (IF/ICC), MYL3 localizes to cytoplasmic sarcomeric structures; validate specificity using appropriate controls.
• ELISA performance may require optimization of coating antigen and antibody dilution; cross-reactivity with mouse and rat MYL3 allows multispecies studies.
• Always validate the antibody in the relevant sample system and application context before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides IVD manufacturers, research laboratories, and diagnostic developers with seamless access to high-quality raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For cardiac muscle and myosin research, our one-stop supply chain supports rare target raw-material sourcing, streamlining your path from concept to clinic. Let CamelBio bridge your procurement gaps with reliable, research-grade reagents.