Anti-MYL2 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P10916

Core Product Specifications and Parameters

Biological Background: MYL2 Function and Localization

• MYL2 encodes ventricular/cardiac myosin regulatory light chain 2 (MLC-2v), also known as cardiac myosin light chain 2 (MLC-2), a key component of the sarcomeric thick filament in cardiac and slow skeletal muscle.
• Functions as a contractile protein crucial for heart development; upon phosphorylation, it regulates cross-bridge cycling kinetics, increases myosin lever arm stiffness, and enhances calcium sensitivity of contraction force. Related references: PMID:23365102 PMID:32453731
• Localizes to the A band of sarcomeres within myofibrils in the cytoplasm of cardiomyocytes.
• Highly expressed in type I (slow-twitch) muscle fibers.
• Associated with familial hypertrophic cardiomyopathy (CMH10) and myofibrillar myopathy (MFM12) when mutated.
• Contains calcium-binding EF-hand motifs and is regulated by phosphorylation and methylation, suggesting metal-dependent regulatory mechanisms.

Experimental Guidance and Technical Tips

• The immunogen used (recombinant protein covering amino acids 1–166) suggests the antibody recognizes an epitope within the N-terminal region of MYL2, which may detect the full-length ~19 kDa protein in western blotting; a band near this molecular weight is expected in cardiac and slow skeletal muscle lysates.
• Due to high sequence conservation, cross-reactivity with mouse and rat orthologs is confirmed; consider validation in the relevant species' tissue extracts.
• For immunofluorescence (IF-P), anticipate sarcomeric A band staining in cardiac muscle sections; appropriate fixation and permeabilization should be optimized.
• This antibody is applicable in ELISA; consider its use in quantitative assays targeting MYL2 in serum or tissue homogenates, but validate with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering one-stop access to validated antibodies, recombinant proteins, and ancillary reagents. Our MYL2 polyclonal antibody supports research in cardiac muscle physiology and cardiomyopathy. We supply optimized monoclonal and polyclonal antibodies, antigen pairs, and bulk reagents to streamline your diagnostic development from concept to clinic.