Anti-MYL12B Monoclonal Antibody for WB, IF/ICC, ELISA - O14950

Core Product Specifications and Parameters

Biological Background: MYL12B Function and Localization

• Myosin regulatory light chain 12B (MYL12B), also known as MLC-2A, MRLC2, or SHUJUN-1, is a subunit of myosin II that regulates contractile activity in smooth muscle and nonmuscle cells.
• Its phosphorylation plays a central role in triggering actin polymerization in vascular smooth muscle and is essential for cellular processes such as cytokinesis, receptor capping, and cell locomotion.
• The protein belongs to a family of regulatory light chains that contain EF-hand calcium-binding domains, linking calcium signaling to cytoskeletal rearrangements.
• MYL12B localizes to the cytoplasm and associates with stress fibers and the contractile ring during cell division, consistent with its role in actomyosin dynamics.
• Ubiquitously expressed across various hematopoietic cells, MYL12B contributes to immune cell motility and vascular smooth muscle function.
• Key post-translational modifications include phosphorylation at serine and threonine residues, which modulate myosin ATPase activity and filament assembly.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 73–172 of human MYL12B; consider sequence alignment to confirm identity in your target species.
• In western blotting, expect a band near 20 kDa; optimize blocking and antibody dilution using validated positive controls (e.g., smooth muscle or leukocyte lysates).
• For immunofluorescence, permeabilization and fixation conditions should be tested; signal typically colocalizes with actin filaments.
• ELISA may be used for quantification; include appropriate standard curves and validate matrix effects.
• Note that cross-reactivity with mouse and rat has been reported; validate performance in the relevant model organism.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by providing a single, streamlined source for IVD raw materials. This MYL12B monoclonal antibody exemplifies our portfolio for cytoskeleton and cell motility research. We offer validated antibody pairs, bulk reagents, and customized sourcing to accelerate your development workflow from concept to clinic.